Karakuş Selcan, Insel Mert Akın, Kahyaoğlu İbrahim Mizan, Albayrak İnci, Ustun-Alkan Fulya
Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, 34320 Avcilar, Istanbul, Turkey.
Department of Chemical Engineering, Yıldız Technical University, 34210 Istanbul, Turkey.
Cellulose (Lond). 2022;29(7):3871-3887. doi: 10.1007/s10570-022-04522-9. Epub 2022 Mar 22.
Currently, researchers are focusing on the development of nano-additive preservatives during the worldwide COVID-19 pandemic. This research aimed to constitute a small sized preservative nano-formulation which emerges from the biopolymer carboxymethyl cellulose (a green stabilizing agent) and hydromagnesite stromatolite (a fossilized natural additive). In this study, we investigated the optimization of the experimental design of carboxymethyl cellulose/hydromagnesite stromatolite (CMC/HS) bio-nanocomposites using a green and one-step sonochemical method at room temperature. In addition, we constructed a mathematical model which relates the intrinsic viscosity with all operating variables, and we carried out statistical error analysis to assess the validity of the proposed model. The characterization and chemical functional groups of CMC/HS bio-nanocomposites were determined by different advanced techniques such as SEM, HRTEM, DLS, FTIR, XRD, and BET. The challenge test was used to show the preservative efficacy of CMC/HS bio-nanocomposites against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, and Aspergillus brasiliensis. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltrazolium bromide (MTT) assay was performed on L929 cells to evaluate the in vitro cytotoxicity of CMC/HS bio-nanocomposites. According to the results, we showed that the synthesized CMC/HS bio-nanocomposites have no cytototoxic effects on L929 fibroblast cells and could be considered to be an alternative green nano-additive preservative against pathogenic microorganisms.
目前，在全球新冠疫情期间，研究人员正专注于纳米添加剂防腐剂的研发。本研究旨在构建一种小型防腐剂纳米制剂，该制剂由生物聚合物羧甲基纤维素（一种绿色稳定剂）和水菱镁矿叠层石（一种化石天然添加剂）制成。在本研究中，我们采用绿色一步室温超声化学法研究了羧甲基纤维素/水菱镁矿叠层石（CMC/HS）生物纳米复合材料实验设计的优化。此外，我们构建了一个将特性粘度与所有操作变量相关联的数学模型，并进行了统计误差分析以评估所提模型的有效性。通过扫描电子显微镜（SEM）、高分辨率透射电子显微镜（HRTEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和比表面积分析仪（BET）等不同先进技术对CMC/HS生物纳米复合材料的特性和化学官能团进行了测定。采用挑战试验来展示CMC/HS生物纳米复合材料对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、白色念珠菌和巴西曲霉的防腐效果。对L929细胞进行了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定，以评估CMC/HS生物纳米复合材料的体外细胞毒性。根据结果，我们表明合成的CMC/HS生物纳米复合材料对L929成纤维细胞没有细胞毒性作用，可被视为一种替代的绿色纳米添加剂防腐剂来对抗致病微生物。
