Tevlek Atakan, Hosseinian Pezhman, Ogutcu Cansel, Turk Mustafa, Aydin Halil Murat
Bioengineering Division, Institute of Science and Engineering, Hacettepe University, Ankara, Turkey.
Nanotechnology and Nanomedicine Division, Institute of Science and Engineering, Hacettepe University, Ankara, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:316-324. doi: 10.1016/j.msec.2016.11.082. Epub 2016 Nov 27.
This study aims to establish a facile protocol for the preparation of a bi-layered poly(glycerol-sebacate) (PGS)/β-tricalcium phosphate (β-TCP) construct and to investigate its potential for bone-soft tissue engineering applications. The layered structure was prepared by distributing the ceramic particles within a prepolymer synthesized in a microwave reactor followed by a cross-linking of the final construct in vacuum (<10mbar). The vacuum stage led to the separation of cross-linked elastomer (top) and ceramic (bottom) phases. Results showed that addition of β-TCP particles to the elastomer matrix after the polymerization led to an increase in compression strength (up to 14±2.3MPa). Tensile strength (σ), Young's modulus (E), and elongation at break (%) values were calculated as 0.29±0.03MPa and 0.21±0.03; 0.38±0.02 and 1.95±0.4; and 240±50% and 24±2% for PGS and PGS/β-TCP bi-layered constructs, respectively. Morphology was characterized by using Scanning Electron Microscopy (SEM) and micro-computed tomography (μ-CT). Tomography data revealed an open porosity of 35% for the construct, mostly contributed from the ceramic phase since the elastomer side has no pore. Homogeneous β-TCP distribution within the elastomeric structure was observed. Cell culture studies confirmed biocompatibility with poor elastomer-side and good bone-side cell attachment. In a further study to investigate the osteogenic properties, the construct were loaded with BMP-2 and/or TGF-β1. The PGS/β-TCP bi-layered constructs with improved mechanical and biological properties have the potential to be used in bone-soft tissue interface applications where soft tissue penetration is a problem.
本研究旨在建立一种简便的制备双层聚（甘油癸二酸酯）（PGS）/β-磷酸三钙（β-TCP）构建体的方案，并研究其在骨-软组织工程应用中的潜力。通过将陶瓷颗粒分布在微波反应器中合成的预聚物中，然后在真空（<10毫巴）中对最终构建体进行交联来制备层状结构。真空阶段导致交联弹性体（顶部）和陶瓷（底部）相分离。结果表明，聚合后向弹性体基质中添加β-TCP颗粒会导致压缩强度增加（高达14±2.3兆帕）。PGS和PGS/β-TCP双层构建体的拉伸强度（σ）、杨氏模量（E）和断裂伸长率（%）值分别计算为0.29±0.03兆帕和0.21±0.03；0.38±0.02和1.95±0.4；以及240±50%和24±2%。通过扫描电子显微镜（SEM）和微计算机断层扫描（μ-CT）对形态进行表征。断层扫描数据显示该构建体的开孔孔隙率为35%，主要由陶瓷相贡献，因为弹性体一侧没有孔隙。观察到β-TCP在弹性体结构内分布均匀。细胞培养研究证实了其生物相容性，弹性体一侧细胞附着较差，而骨一侧细胞附着良好。在进一步研究其成骨特性时，将构建体加载BMP-2和/或TGF-β1。具有改善的机械和生物学性能的PGS/β-TCP双层构建体有潜力用于软组织穿透是一个问题的骨-软组织界面应用中。
