Cerniglia C E, Campbell W L, Freeman J P, Evans F E
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079.
Appl Environ Microbiol. 1989 Sep;55(9):2275-9. doi: 10.1128/aem.55.9.2275-2279.1989.
The metabolism of phenanthrene by the fungus Cunninghamella elegans was investigated. Kinetic experiments using [9-14C]phenanthrene showed that after 72 h, 53% of the total radioactivity was associated with a glucoside conjugate of 1-hydroxyphenanthrene (phenanthrene 1-O-beta-glucose). This metabolite was isolated by reversed-phase high-performance liquid chromatography and characterized by the application of UV absorption, 1H nuclear magnetic resonance, and mass spectral techniques. The results show that aromatic ring oxidation followed by glucosylation is a predominant pathway in the metabolism of the polycyclic aromatic hydrocarbon phenanthrene by C. elegans.
研究了真菌雅致小克银汉霉对菲的代谢。使用[9-¹⁴C]菲进行的动力学实验表明，72小时后，总放射性的53%与1-羟基菲（菲1-O-β-葡萄糖）的葡糖苷缀合物相关。该代谢物通过反相高效液相色谱法分离，并通过紫外吸收、¹H核磁共振和质谱技术进行表征。结果表明，芳香环氧化后进行糖基化是雅致小克银汉霉代谢多环芳烃菲的主要途径。
