Cerniglia C E, Freeman J P, White G L, Heflich R H, Miller D W
Appl Environ Microbiol. 1985 Sep;50(3):649-55. doi: 10.1128/aem.50.3.649-655.1985.
Nitropolycyclic aromatic hydrocarbons are ubiquitous environmental pollutants, many of which are potent mutagens in bacterial and mammalian cells and carcinogenic to rodents. In this study, we investigated the fungal metabolism of 1-nitropyrene and determined the mutagenic activity of the metabolites toward Salmonella typhimurium TA98, TA98NR, and TA100. Cunninghamella elegans metabolized 1-nitropyrene to form glucoside conjugates of 6-hydroxy-1-nitropyrene and 8-hydroxy-1-nitropyrene. The metabolites were isolated by reversed-phase high-pressure liquid chromatography and characterized by application of UV absorption, 1H-nuclear magnetic resonance, and mass spectroscopy. Mutagenicity assays performed on samples extracted from incubations of C. elegans with 1-nitropyrene indicated that mutagenic activity decreased with time. Consistent with the loss in mutagenic activity, the glucoside conjugates of 6- and 8-hydroxy-1-nitropyrene were nonmutagenic in the Salmonella reversion assay. The results indicate that the fungus C. elegans metabolizes 1-nitropyrene to detoxified products.
硝基多环芳烃是普遍存在的环境污染物，其中许多在细菌和哺乳动物细胞中是强效诱变剂，对啮齿动物具有致癌性。在本研究中，我们研究了1-硝基芘的真菌代谢，并确定了代谢产物对鼠伤寒沙门氏菌TA98、TA98NR和TA100的诱变活性。雅致小克银汉霉将1-硝基芘代谢形成6-羟基-1-硝基芘和8-羟基-1-硝基芘的葡萄糖苷缀合物。通过反相高压液相色谱法分离代谢产物，并通过紫外吸收、1H-核磁共振和质谱进行表征。对雅致小克银汉霉与1-硝基芘孵育后的样品进行的致突变性试验表明，诱变活性随时间降低。与诱变活性的丧失一致，6-和8-羟基-1-硝基芘的葡萄糖苷缀合物在沙门氏菌回复试验中无诱变作用。结果表明，雅致小克银汉霉将1-硝基芘代谢为解毒产物。
