Zhang Na, Liu Chao, Sun Tie-Min, Ran Xiao-Ku, Kang Ting-Guo, Dou De-Qiang
a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
b College of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang 110016 , China.
J Asian Nat Prod Res. 2017 Jan;19(1):35-41. doi: 10.1080/10286020.2016.1247351.
In the present study, two new compounds, together with six known compounds, were isolated from rhizome of Atractylodes macrocephala Koidz by a series of silica gel, ODS column chromatography, and preparative HPLC. Their structures were characterized as atractylenolide II (1), atractylenolide I (2), biepiasterolid (3), isoatractylenolide I (4), atractylenolide III (5), 3β-acetoxyl atractylenolide I (6), (4E,6E,12E)-tetradeca-4,6,12-triene-8,10-diyne-13,14-triol (7), (3S,4E,6E,12E)-1-acetoxy-tetradeca-4,6,12-triene-8,10-diyne-3,14-diol (8) on the basis of 1D, 2D NMR, and circular dichroism analyses. Among them, compounds 6 and 8 were novel compounds. In addition, their neuroprotective activity against MPP-induced SH-SY5Y cells was evaluated by MTT colorimetry. The results showed that all these compounds have definite protective effect on MPP-induced SH-SY5Y cells.
在本研究中，通过一系列硅胶、ODS柱色谱和制备型高效液相色谱，从白术根茎中分离出两种新化合物以及六种已知化合物。基于一维、二维核磁共振和圆二色性分析，它们的结构被鉴定为苍术烯内酯II（1）、苍术烯内酯I（2）、双表甾醇（3）、异苍术烯内酯I（4）、苍术烯内酯III（5）、3β - 乙酰氧基苍术烯内酯I（6）、（4E，6E，12E）-十四碳-4,6,12-三烯-8,10-二炔-13,14-二醇（7）、（3S，4E，6E，12E）-1-乙酰氧基-十四碳-4,6,12-三烯-8,10-二炔-3,14-二醇（8）。其中，化合物6和8是新化合物。此外，采用MTT比色法评估了它们对MPP诱导的SH-SY5Y细胞的神经保护活性。结果表明，所有这些化合物对MPP诱导的SH-SY5Y细胞均具有一定的保护作用。
