Singh Priya, Rohilla Manish, Dey Pranab
Department of Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, Punjab and Haryana, India.
Department of Cytopathology and Gynecological Pathology, Postgraduate Institute of Medical Education and Research, Chandigarh, Punjab and Haryana, India.
J Cytol. 2016 Oct-Dec;33(4):187-191. doi: 10.4103/0970-9371.190444.
In this paper, we have compared the cytomorphologic characteristics of liquid-based preparation (LBP) [SurePath (SP)] cytology and conventional smear (CP) preparations on fine-needle aspiration (FNAC) material by a semi-quantitative scoring system for cases of lymphadenopathy.
In this prospective study, a total of 52 consecutive cases of FNAC of lymphadenopathy were included. The first pass was used for CP followed by LBP with the help of SP technique. The smears were independently compared and assessed by two observers (PS and PD).
The semiquantitative grading was compared in two groups by Wilcoxon signed-rank test. The background information, cell architecture, pleomorphism, nuclear and cytoplasmic details, and three-dimensional structures were significantly different in LBP and CP smears.
Liquid-based cytology (LBC) is a relatively simple technique, which exhibits good nuclear and cytoplasmic details with the absence of obscuring background material. Even the number of slides and area per slide to be screened were less than the conventional preparation but caution must be applied to interpret the slides and secure a diagnosis, especially if LBC is the first and only method applied for diagnosis.
在本文中，我们通过半定量评分系统，比较了液基制片（LBP）[SurePath（SP）]细胞学和传统涂片（CP）制片在淋巴结病细针穿刺抽吸（FNAC）材料上的细胞形态学特征。
在这项前瞻性研究中，共纳入52例连续的淋巴结病FNAC病例。首次穿刺用于CP，随后借助SP技术进行LBP。涂片由两名观察者（PS和PD）独立比较和评估。
通过Wilcoxon符号秩检验比较两组的半定量分级。LBP和CP涂片在背景信息、细胞结构、多形性、细胞核和细胞质细节以及三维结构方面存在显著差异。
液基细胞学（LBC）是一种相对简单的技术，它能呈现良好的细胞核和细胞质细节，且无背景材料遮挡。即使需要筛查的玻片数量和每张玻片的面积都少于传统制片，但在解读玻片并做出诊断时必须谨慎，特别是当LBC是用于诊断的首个且唯一方法时。
