Jiminez Janelle A, Uwiera Trina C, Abbott D Wade, Uwiera Richard R E, Inglis G Douglas
Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1 Canada.
Department of Agricultural Food and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5 Canada.
Gut Pathog. 2016 Dec 22;8:67. doi: 10.1186/s13099-016-0149-6. eCollection 2016.
Identifying the connection among diet, the intestinal microbiome, and host health is currently an area of intensive research, but the potential of dietary fiber (DF) consumption to ameliorate intestinal inflammation has not been extensively studied. We examined the impacts of the DFs, wheat bran (WB) and resistant starch (RS) on host enteric health. A murine model of acute Th1/Th17 colitis (i.e. incited by ) was used.
Diets enriched with RS increased weight gain in mice inoculated with compared to mice consuming a conventional control (CN) diet. Short-chain fatty acid (SCFA) quantities in the cecum and distal colon were higher in mice consuming DFs, and these mice exhibited higher butyrate concentrations in the distal colon during inflammation. Histopathologic scores of inflammation in the proximal colon on day 14 post-inoculation (p.i.) (peak infection) and 21 p.i. (late infection) were lower in mice consuming DF-enriched diets compared to the CN diet. Consumption of WB reduced the expression of Th1/Th17 cytokines. As well, the expression of bacterial recognition and response genes such as , , and increased in mice consuming the RS-enriched diets. Furthermore, each diet generated a region-specific bacterial community, suggesting a link between selection for specific bacterial communities, SCFA concentrations, and inflammation in the murine colon.
Collectively, data indicated that the consumption of DF-rich diets ameliorates the effects of -induced enteritis by modifying the host microbiota to increase SCFA production, and bacterial recognition and response mechanisms to promote host health.
确定饮食、肠道微生物群和宿主健康之间的联系是当前一个深入研究的领域，但膳食纤维（DF）摄入改善肠道炎症的潜力尚未得到广泛研究。我们研究了膳食纤维、麦麸（WB）和抗性淀粉（RS）对宿主肠道健康的影响。使用了急性Th1/Th17结肠炎的小鼠模型（即由……引发）。
与食用传统对照（CN）饮食的小鼠相比，富含RS的饮食使接种……的小鼠体重增加。食用膳食纤维的小鼠盲肠和远端结肠中的短链脂肪酸（SCFA）量更高，并且这些小鼠在炎症期间远端结肠中的丁酸盐浓度更高。与CN饮食相比，食用富含DF饮食的小鼠在接种后第14天（感染高峰期）和接种后第21天（感染后期）近端结肠的炎症组织病理学评分更低。食用WB可降低Th1/Th17细胞因子的表达。同样，在食用富含RS饮食的小鼠中，诸如……、……和……等细菌识别和反应基因的表达增加。此外，每种饮食都产生了区域特异性细菌群落，表明在小鼠结肠中特定细菌群落的选择、SCFA浓度和炎症之间存在联系。
总体而言，数据表明食用富含DF的饮食通过改变宿主微生物群以增加SCFA产生以及细菌识别和反应机制来促进宿主健康，从而改善……诱导的肠炎的影响。
