蛋白水解酶在培养的干细胞非预期分化中可能发挥的作用。
The likely role of proteolytic enzymes in unwanted differentiation of stem cells in culture.
作者信息
Penna Vanessa, Lipay Monica Vn, Duailibi Monica T, Duailibi Silvio E
机构信息
Graduate Translational Surgery Program, Paulista School of Medicine, Federal University of Sao Paulo (EPM/UNIFESP), SP, Brazil; CTCMol, Center of Cellular & Molecular Therapy, São Paulo, SP, Brazil; Graduate Translational Surgery Program, Paulista School of Medicine, Federal University of Sao Paulo (EPM/UNIFESP), SP, Brazil; CTCMol, Center of Cellular & Molecular Therapy, São Paulo, SP, Brazil.
Department of Morphology & Basic Pathology, Jundiaí Faculty of Medicine, São Paulo, SP, Brazil; Medicine Department, Endocrinology Division, EPM/UNIFESP, Sao Paulo, SP, Brazil; Department of Morphology & Basic Pathology, Jundiaí Faculty of Medicine, São Paulo, SP, Brazil; Medicine Department, Endocrinology Division, EPM/UNIFESP, Sao Paulo, SP, Brazil.
出版信息
Future Sci OA. 2015 Nov 1;1(3):FSO28. doi: 10.4155/fso.15.26. eCollection 2015 Nov.
Tissue engineering aims at developing the necessary technological strategies for replacement or regeneration tissues. However, the number of cells required is much greater than the number obtained from a cell source. Expanding the cells' number in cell culture for a long period is required until the necessary amount of cells is obtained. While in culture, cells often go unwanted differentiation. Little attention has been given to the use of proteolytic enzymes in cell passage. Review the importance of extracellular matrix and surface proteins for cell behavior and the possible mechanisms of cellular changes that may occur due to the use of proteolytic enzymes is an essential issue. Possible alternative to replace these enzymes in cell passage has also been developed.
组织工程旨在开发用于组织替代或再生的必要技术策略。然而，所需的细胞数量远远大于从细胞源获得的细胞数量。需要在细胞培养中长期扩增细胞数量，直到获得所需数量的细胞。在培养过程中，细胞常常会发生不需要的分化。在细胞传代过程中使用蛋白水解酶的情况很少受到关注。审视细胞外基质和表面蛋白对细胞行为的重要性以及由于使用蛋白水解酶可能发生的细胞变化的潜在机制是一个至关重要的问题。在细胞传代中替代这些酶的可能替代方法也已被开发出来。
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