Grant D, Long W F, Moffat C F, Williamson F B
Department of Biochemistry, University of Aberdeen, Marischal College, Scotland, U.K.
Biochem J. 1989 Aug 1;261(3):1035-8. doi: 10.1042/bj2611035.
Analysis by i.r. spectroscopy of natural and chemically modified heparins suggests that the N-sulphonate group of these polymers may vibrationally absorb radiation at two distinctly different frequencies. This property may reflect the existence of different conformational states important in the biological activities of the polymers. Examination of carboxylate- and N-acetyl-group absorbances of these polymers accords with the possibility that one of these polymer conformational states involves interaction between juxtaposed N-sulphonate and carboxylate groups. In heparin bearing large quantities of artificially introduced N-acetyl groups, an interaction between carboxylate and N-acetyl groups may also occur.
对天然肝素和化学修饰肝素进行红外光谱分析表明，这些聚合物的N-磺酸根基团可能会在两个明显不同的频率下发生振动吸收辐射。这一特性可能反映了在聚合物生物活性中重要的不同构象状态的存在。对这些聚合物的羧基和N-乙酰基吸光度的研究符合以下可能性，即这些聚合物构象状态之一涉及并列的N-磺酸根基团和羧基之间的相互作用。在含有大量人工引入的N-乙酰基的肝素中，羧基和N-乙酰基之间也可能发生相互作用。
