“回路时代”的突触生物学——通向分子连接组学的路径
Synapse biology in the 'circuit-age'-paths toward molecular connectomics.
作者信息
Schreiner Dietmar, Savas Jeffrey N, Herzog Etienne, Brose Nils, de Wit Joris
机构信息
Biozentrum, University of Basel, Klingelbergstraße 50-70, 4056 Basel, Switzerland; Institute of Neuroanatomy and Cell Biology, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Department of Neurology, Northwestern University, Feinberg School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611, USA.
出版信息
Curr Opin Neurobiol. 2017 Feb;42:102-110. doi: 10.1016/j.conb.2016.12.004. Epub 2016 Dec 26.
The neural connectome is a critical determinant of brain function. Circuits of precisely wired neurons, and the features of transmission at the synapses connecting them, are thought to dictate information processing in the brain. While recent technological advances now allow to define the anatomical and functional neural connectome at unprecedented resolution, the elucidation of the molecular mechanisms that establish the precise patterns of connectivity and the functional characteristics of synapses has remained challenging. Here, we describe the power and limitations of genetic approaches in the analysis of mechanisms that control synaptic connectivity and function, and discuss how recent methodological developments in proteomics might be used to elucidate the molecular synaptic connectome that is at the basis of the neural connectome.
神经连接组是脑功能的关键决定因素。精确布线的神经元回路以及连接它们的突触处的传递特征，被认为决定了大脑中的信息处理。尽管最近的技术进步现在允许以前所未有的分辨率定义解剖学和功能性神经连接组，但阐明建立精确连接模式和突触功能特征的分子机制仍然具有挑战性。在这里，我们描述了遗传方法在分析控制突触连接性和功能的机制中的优势和局限性，并讨论了蛋白质组学中最近的方法学进展如何可能用于阐明作为神经连接组基础的分子突触连接组。
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