Krzych-Fałta Edyta, Furmańczyk Konrad, Samoliński Bolesław
Unit of Environmental Hazard Prevention and Allergology, Faculty of Health Science, Medical University of Warsaw, Warsaw, Poland.
Unit of Environmental Hazard Prevention and Allergology, Faculty of Health Science, Medical University of Warsaw, Warsaw, Poland; Chair of Applied Mathematics, Faculty of Applied Informatics and Mathematics, Warsaw University of Life Sciences, Warsaw, Poland.
Postepy Dermatol Alergol. 2016 Dec;33(6):464-468. doi: 10.5114/pdia.2016.61339. Epub 2016 Dec 2.
Nasal provocation testing involves an allergen-specific local reaction of the nasal mucosa to the administered allergen.
To determine the most objective nasal occlusion assessment technique that could be used in nasal provocation testing.
A total of 60 subjects, including 30 patients diagnosed with allergy to common environmental allergens and 30 healthy subjects were enrolled into the study. The method used in the study was a nasal provocation test with an allergen, with a standard dose of a control solution and an allergen (5,000 SBU/ml) administered using a calibrated atomizer into both nostrils at room temperature. Early-phase nasal mucosa response in the early phase of the allergic reaction was assessed via acoustic rhinometry, optical rhinometry, nitric oxide in nasal air, and tryptase levels in the nasal lavage fluid.
In estimating the homogeneity of the average values, the Levene's test was used and receiver operating characteristic curves were plotted for all the methods used for assessing the nasal provocation test with an allergen. Statistically significant results were defined for < 0.05. Of all the objective assessment techniques, the most sensitive and characteristic ones were the optical rhinometry techniques (specificity = 1, sensitivity = 1, AUC = 1, PPV = 1, NPV = 1).
The techniques used showed significant differences between the group of patients with allergic rhinitis and the control group. Of all the objective assessment techniques, those most sensitive and characteristic were the optical rhinometry.
鼻激发试验涉及鼻黏膜对所给予变应原的变应原特异性局部反应。
确定可用于鼻激发试验的最客观的鼻阻塞评估技术。
共有60名受试者纳入本研究，其中包括30名被诊断为对常见环境变应原过敏的患者和30名健康受试者。本研究采用的方法是变应原鼻激发试验，在室温下使用校准雾化器将标准剂量的对照溶液和变应原（5000 SBU/ml）注入双侧鼻孔。通过鼻声反射、光学鼻测量法、鼻腔空气中的一氧化氮以及鼻腔灌洗液中的类胰蛋白酶水平评估过敏反应早期阶段的早期鼻黏膜反应。
在估计平均值的同质性时，使用了Levene检验，并为所有用于评估变应原鼻激发试验的方法绘制了受试者工作特征曲线。统计学显著结果定义为P<0.05。在所有客观评估技术中，最敏感和最具特征性的是光学鼻测量法技术（特异性=1，敏感性=1，AUC=1，PPV=1，NPV=1）。
所使用的技术在变应性鼻炎患者组和对照组之间显示出显著差异。在所有客观评估技术中，最敏感和最具特征性的是光学鼻测量法。
