Huang Zebo, Zhang Lan, Zhu Danxia, Shan Xia, Zhou Xin, Qi Lian-Wen, Wu Lirong, Zhu Jun, Cheng Wenfang, Zhang Huo, Chen Yan, Zhu Wei, Wang Tongshan, Liu Ping
Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
Department of Oncology, The Third Affiliated Hospital of Soochow University, 185 Juqian Street, Changzhou, 213003, China.
Cancer Med. 2017 Jan;6(1):109-119. doi: 10.1002/cam4.973. Epub 2016 Dec 30.
Circulating microRNAs (miRNAs) have been used as promising diagnostic biomarkers for esophageal squamous cell carcinoma (ESCC). We performed miRNA expression profiling using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panels from three ESCC pools and one normal control (NC) pool samples. Using qRT-PCR, identified serum miRNAs were further confirmed in training (32 ESCC vs. 32 NCs) and testing stages (108 ESCC vs. 96 NCs). Consequently, five serum miRNAs (miR-20b-5p, miR-28-3p, miR-192-5p, miR-223-3p, and miR-296-5p) were significantly overexpressed in ESCC compared with NCs. The diagnostic value of the 5-miRNA signature was validated by an external cohort (60 ESCC vs. 60 NCs). The areas under the receiver operating characteristic curve (ROC) of the 5-miRNA signature were 0.753, 0.763, and 0.966 for the training, testing, and the external validation stages, respectively. The expression levels of the miRNAs were also determined in tissues, arterial serum, and exosomes. MiR-20b-5p, miR-28-3p, and miR-192-5p were significantly upregulated in ESCC tissues, while miR-296-5p was overexpressed in ESCC serum exosomes. In conclusion, we identified a 5-miRNA signature in serum for the detection of ESCC.
循环微RNA（miRNA）已被用作食管鳞状细胞癌（ESCC）颇具前景的诊断生物标志物。我们使用基于定量逆转录聚合酶链反应（qRT-PCR）的Exiqon检测板，对三个ESCC样本库和一个正常对照（NC）样本库进行了miRNA表达谱分析。通过qRT-PCR，在训练阶段（32例ESCC患者与32例NC患者）和测试阶段（108例ESCC患者与96例NC患者）进一步确认了所鉴定的血清miRNA。结果发现，与NC患者相比，ESCC患者中有五种血清miRNA（miR-20b-5p、miR-28-3p、miR-192-5p、miR-223-3p和miR-296-5p）显著过表达。通过一个外部队列（60例ESCC患者与60例NC患者）验证了5-miRNA标志物的诊断价值。5-miRNA标志物在训练、测试和外部验证阶段的受试者操作特征曲线（ROC）下面积分别为0.753、0.763和0.966。还测定了这些miRNA在组织、动脉血清和外泌体中的表达水平。MiR-20b-5p、miR-28-3p和miR-192-5p在ESCC组织中显著上调，而miR-296-5p在ESCC血清外泌体中过表达。总之，我们鉴定出一种血清中的5-miRNA标志物用于检测ESCC。
