Nielsen K, Petersen S E, Orntoft T
University Institute of Pathology, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1989 Oct;97(10):949-56. doi: 10.1111/j.1699-0463.1989.tb00503.x.
The correlation between stereological estimate of mean nuclear volume and DNA-content was studied in 55 human urinary bladder tumours. The DNA-content was determined by flow cytometry on isolated nuclei stained with ethidium bromide. Trout erythrocytes were used as a biological internal standard providing an accurate determination of the DNA-histogram. An unbiased estimate of the mean nuclear volume (vv) was obtained after standard formaline fixation, paraffin-embedding, sectioning and hematoxylin-eosin staining using the equation vv = pi/3.l3(0). Here l0 is the length of an intercept measured in a random direction through a test point, which hit a nucleus. A highly significant correlation was found between vv and mean DNA-content of nuclei (2p = 0.0004). A highly significant correlation was also found between vv and the highest DNA-content present in tumours having cell populations with different DNA content (2p = 0.0016). The mean nuclear volume and the DNA-content also correlated well with the pathologic grade. Although significant the correlations were far from perfect, which indicates that DNA content and mean nuclear volume may provide partly independent biological information. The methods provide objective, unbiased and reproducible data which may improve the possibility of grading and predicting the disease course of human urinary bladder tumours.
在55例人类膀胱肿瘤中研究了平均核体积的体视学估计值与DNA含量之间的相关性。DNA含量通过流式细胞术测定，所用的分离细胞核用溴化乙锭染色。鳟鱼红细胞用作生物内标，以准确测定DNA直方图。在标准福尔马林固定、石蜡包埋、切片和苏木精-伊红染色后，使用公式vv = π/3·l3(0)获得平均核体积（vv）的无偏估计值。这里l0是在随机方向上通过与细胞核相交的测试点测量的截距长度。发现vv与细胞核的平均DNA含量之间存在高度显著的相关性（双尾P = 0.0004）。在具有不同DNA含量细胞群体的肿瘤中，vv与最高DNA含量之间也发现了高度显著的相关性（双尾P = 0.0016）。平均核体积和DNA含量与病理分级也有很好的相关性。尽管相关性显著，但远非完美，这表明DNA含量和平均核体积可能提供部分独立的生物学信息。这些方法提供了客观、无偏且可重复的数据，这可能会提高人类膀胱肿瘤分级和预测疾病进程的可能性。
