IgG、IgM多重等离子体金平台在法国临床队列中用于弓形虫感染血清学诊断及随访的验证
Validation of IgG, IgM multiplex plasmonic gold platform in French clinical cohorts for the serodiagnosis and follow-up of Toxoplasma gondii infection.
作者信息
Pomares Christelle, Zhang Bo, Arulkumar Shylaja, Gonfrier Geraldine, Marty Pierre, Zhao Su, Cheng Steven, Tang Meijie, Dai Hongjie, Montoya Jose G
机构信息
Division of Infectious Diseases, Stanford University, Stanford, California 94305, USA; Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, California, USA; INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes - Université de Nice Sophia Antipolis, Faculté de Médecine, 06204 Nice, Cedex 3, France; Parasitologie-Mycologie, Centre Hospitalier Universitaire l'Archet, CS 23079, 06202 Nice, Cedex 3, France.
Nirmidas Biotech Inc., Palto Alto, CA 94304, USA.
出版信息
Diagn Microbiol Infect Dis. 2017 Mar;87(3):213-218. doi: 10.1016/j.diagmicrobio.2016.09.001. Epub 2016 Sep 8.
We report the use of the multiplexed T. gondii IgG, IgM test on plasmonic gold (pGOLD) platform in the setting of T. gondii infection by analyzing 244 sera from Nice, France (seroconversion, chronically infected, non-infected and newborns serum samples). Results were compared with commercial tests for the detection of IgG and IgM and their overall clinical final interpretation of a complete serological profile. The IgG and IgM test results on the platform were in agreement in, respectively, 95% and 93% with the commercial kits. When comparing with the overall clinical interpretation of the serological profile, the agreement reached 99.5% and 97.7% for IgG and IgM, respectively. This innovative pGOLD platform allows detection of both IgG and IgM simultaneously with only ~1 microliter of serum. The multiplexed IgG/IgM test on pGOLD platform is a strong candidate for its use in the massive screening programs for toxoplasmosis during pregnancy.
我们报告了通过分析来自法国尼斯的244份血清样本（血清转化、慢性感染、未感染及新生儿血清样本），在弓形虫感染情况下，在等离子体金（pGOLD）平台上使用多重弓形虫IgG、IgM检测的情况。将结果与用于检测IgG和IgM的商业检测方法以及对完整血清学谱的总体临床最终解释进行了比较。该平台上的IgG和IgM检测结果与商业试剂盒的一致性分别为95%和93%。与血清学谱的总体临床解释相比，IgG和IgM的一致性分别达到99.5%和97.7%。这种创新的pGOLD平台仅需约1微升血清就能同时检测IgG和IgM。pGOLD平台上的多重IgG/IgM检测是用于孕期弓形虫病大规模筛查项目的有力候选方法。
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