通过纳升高效液相色谱与高分辨率质谱联用对插管新生儿的呼出气冷凝物进行分析。

Exhaled breath condensate analysis from intubated newborns by nano-HPLC coupled to high resolution MS.

作者信息

Kononikhin A S, Starodubtseva N L, Chagovets V V, Ryndin A Y, Burov A A, Popov I A, Bugrova A E, Dautov R A, Tokareva A O, Podurovskaya Y L, Ionov O V, Frankevich V E, Nikolaev E N, Sukhikh G T

机构信息

V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Moscow Institute of Physics and Technology, Moscow, Russia.

V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; Moscow Institute of Physics and Technology, Moscow, Russia.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 15;1047:97-105. doi: 10.1016/j.jchromb.2016.12.036. Epub 2016 Dec 23.

DOI:10.1016/j.jchromb.2016.12.036

PMID:28040456

Abstract

Invasiveness of examination and therapy methods is a serious problem for intensive care and nursing of premature infants. Exhaled breath condensate (EBC) is the most attractive biofluid for non-invasive methods development in neonatology for monitoring the status of intubated infants. The aim of the study was to propose an approach for EBC sampling and analysis from mechanically ventilated neonates. EBC collection system with good reproducibility of sampling was demonstrated. Discovery-based proteomic and metabolomic studies were performed using nano-HPLC coupled to high resolution MS. Label-free semi-quantitative data were compared for intubated neonates with congenital pneumonia (12 infants) and left-sided congenital diaphragmatic hernia (12 infants) in order to define disease-specific features. Totally 119 proteins and 164 metabolites were found. A number of proteins and metabolites that can act as potential biomarkers of respiratory diseases were proposed and require further validation.

摘要

检查和治疗方法的侵入性是早产儿重症监护和护理中的一个严重问题。呼出气冷凝物（EBC）是新生儿学中开发用于监测插管婴儿状况的非侵入性方法最具吸引力的生物流体。本研究的目的是提出一种从机械通气新生儿中采集和分析EBC的方法。展示了具有良好采样重现性的EBC采集系统。使用与高分辨率质谱联用的纳米高效液相色谱进行了基于发现的蛋白质组学和代谢组学研究。为了确定疾病特异性特征，对患有先天性肺炎的插管新生儿（12例婴儿）和左侧先天性膈疝的插管新生儿（12例婴儿）的无标记半定量数据进行了比较。总共发现了119种蛋白质和164种代谢物。提出了许多可作为呼吸系统疾病潜在生物标志物的蛋白质和代谢物，需要进一步验证。