Tavalaee Marziyeh, Kiani-Esfahani Abbas, Nasr-Esfahani Mohammad Hossein
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan Fertility and Infertility Center, Isfahan, Iran.
Cell J. 2017 Winter;18(4):588-596. doi: 10.22074/cellj.2016.4725. Epub 2016 Sep 26.
The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development.
In this experimental study, semen samples were collected from 35 intra-cytoplasmic sperm injection (ICSI) candidates and analyzed according to World Health Organization criteria (2010). Each sample was divided into two parts. The first part was processed for insemination by density-gradient centrifugation (DGC) and the second part was prepared for assessment of sperm morphology (Papanicolaou staining), DNA fragmentation [transferase dUTP nick end labeling (TUNEL)], and three Sperm-borne oocyte-activating factor (s) (SOAFs)-PLCζ, PAWP, and TR-KIT.
Significant positive correlations existed between the percentages of PLCζ, PAWP, and TR-KIT with fertilization rate. In addition, significant negative correlations existed between the percentage of DNA fragmentation with the percentages of PLCζ and PAWP. We did not find a relationship between percentages of PLCζ, PAWP, and TR-KIT with embryo quality and pregnancy rate (P>0.05). There was a significant negative correlation between percentage of DNA fragmentation with fertilization and embryo quality.
Oocyte activation was associated with the studied sperm factors (PAWP, PLCζ, and TR-KIT). These factors might hold the potential to be considered as diagnostic factors in the assessment of semen samples to evaluate their potential to induce oocyte activation. In addition, we observed a significant association between DNA fragmentation with fertilization, as well as embryo quality and expression of PAWP and PLCζ, which indicated that men with high degrees of DNA fragmentation might require artificial oocyte activation. Whether such action should take place, and its cost and benefits should be evaluated in the future.
本研究旨在同时评估三种潜在因子[顶体后鞘WW结构域结合蛋白（PAWP）、磷脂酶Cζ（PLCζ）和截短型kit受体（TR-KIT）]作为卵母细胞激活候选因子的表达与受精率及早期胚胎发育之间的关联。
在本实验研究中，从35名接受胞浆内单精子注射（ICSI）的候选者中采集精液样本，并根据世界卫生组织标准（2010年）进行分析。每个样本分为两部分。第一部分通过密度梯度离心（DGC）处理用于授精，第二部分用于评估精子形态（巴氏染色）、DNA片段化[末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）]以及三种精子携带的卵母细胞激活因子（SOAFs）——PLCζ、PAWP和TR-KIT。
PLCζ、PAWP和TR-KIT的百分比与受精率之间存在显著正相关。此外，DNA片段化百分比与PLCζ和PAWP的百分比之间存在显著负相关。我们未发现PLCζ、PAWP和TR-KIT的百分比与胚胎质量及妊娠率之间存在关联（P>0.05）。DNA片段化百分比与受精及胚胎质量之间存在显著负相关。
卵母细胞激活与所研究的精子因子（PAWP、PLCζ和TR-KIT）相关。这些因子可能有潜力被视为评估精液样本以评估其诱导卵母细胞激活潜力的诊断因子。此外，我们观察到DNA片段化与受精以及胚胎质量和PAWP及PLCζ的表达之间存在显著关联，这表明DNA片段化程度高的男性可能需要人工卵母细胞激活。未来应评估是否应采取这种措施及其成本和益处。
