Liu Huixin, Liu Wei, Hou Xuexia, Zhang Lin, Hao Qin, Wan Kanglin
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
Biomed Res Int. 2016;2016:1327320. doi: 10.1155/2016/1327320. Epub 2016 Nov 15.
The 41 kD flagellin of is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease. Here, we chose 89 strains in China, amplified the gene encoding the 41 kD flagellin, and compared the sequences. The results showed that genetic diversity presented in the 41 kD flagellin genes of all 89 strains among the four genotypes of , especially in the genotype of . Some specific mutation sites for each genotype of the 41 kD flagellin genes were found, which could be used for genotyping strains in China. Human B-cell epitope analysis showed that thirteen of 15 nonsynonymous mutations occurred in the epitope region of 41 kD flagellin and thirty of 42 B-cell epitopes were altered due to all 13 nonsynonymous mutations in the epitope region, which may affect the function of the antigen. Nonsynonymous mutations and changed human B-cell epitopes exist in 41 kD flagellin of strains; these changes should be considered in serodiagnosis of Lyme disease.
的41kD鞭毛蛋白是周质鞭毛丝核心的主要成分，是莱姆病早期血清学诊断的良好候选物。在此，我们选取了中国的89株菌株，扩增了编码41kD鞭毛蛋白的基因，并比较了序列。结果表明，在的四种基因型中，所有89株菌株的41kD鞭毛蛋白基因均存在遗传多样性，尤其是在基因型中。发现了41kD鞭毛蛋白基因各基因型的一些特异性突变位点，可用于中国菌株的基因分型。人B细胞表位分析表明，15个非同义突变中的13个发生在41kD鞭毛蛋白的表位区域，42个B细胞表位中的30个因表位区域的所有13个非同义突变而改变，这可能影响抗原的功能。菌株的41kD鞭毛蛋白存在非同义突变和改变的人B细胞表位；在莱姆病的血清学诊断中应考虑这些变化。
