桃金娘科果实可保护 HepG2 细胞免受四氯化碳诱导的毒性作用。
(Myrtaceae) fruits protect HEPG2 cells against carbon tetrachloride-induced toxicity.
作者信息
de Oliveira Fernandes Thaís, de Ávila Renato Ivan, de Moura Soraia Santana, de Almeida Ribeiro Gerlon, Naves Maria Margareth Veloso, Valadares Marize Campos
机构信息
Laboratório de Nutrição Experimental, Faculdade de Nutrição, Universidade Federal de Goiás, rua 227, quadra 68, s/n, Setor Leste Universitário, 74.605-080 Goiânia, GO, Brazil.
Laboratório de Farmacologia e Toxicologia Celular - FarmaTec, Faculdade de Farmácia, Universidade Federal de Goiás, rua 240 esquina com 5ª Avenida, s/n, Setor Universitário, 74.605-220 Goiânia, GO, Brazil.
出版信息
Toxicol Rep. 2014 Dec 16;2:184-193. doi: 10.1016/j.toxrep.2014.11.018. eCollection 2015.
(Myrtaceae) is an antioxidant compounds-rich Brazilian fruit popularly known as . In view of this, it was evaluated the hepatoprotective effects of pulp (GPE) or peel/seed (GPSE) hydroalcoholic extracts of on injured liver-derived HepG2 cells by CCl (4 mM). The results showed the presence of total phenolic in GPSE was (60%) higher when compared to GPE, associated with interesting antioxidant activity using DPPH• assay. Additionally, HPLC chromatograms and thin layer chromatography of GPE and GPSE showed the presence of flavonoids. Pretreatment of HepG2 cells with GPE or GPSE (both at 800-1000 μg/mL) significantly ( < 0.0001) protected against cytotoxicity induced by CCl Additionally, the cells treated with both extracts (both at 1000 μg/mL) showed normal morphology (general and nuclear) contrasting with apoptotic characteristics in the cells only exposed to CCl. In these experiments, GPSE also was more effective than GPE. In addition, CCl induced a marked increase in AST ( < 0.05) and ALT ( < 0.0001) levels, while GPE or GPSE significantly ( < 0.0001) reduced these levels, reaching values found in the control group. In conclusion, the results suggest that fruits exert hepatoprotective effects on HepG2 cells against the CCl-induced toxicity, probably, at least in part, associated with the presence of antioxidant compounds, especially flavonoids.
桃金娘科植物是一种富含抗氧化化合物的巴西水果，俗称[水果名称未给出]。鉴于此，研究了[水果名称未给出]果肉（GPE）或果皮/种子（GPSE）水醇提取物对四氯化碳（4 mM）损伤的肝源性HepG2细胞的肝保护作用。结果表明，与GPE相比，GPSE中总酚含量高（60%），采用DPPH•法检测具有有趣的抗氧化活性。此外，GPE和GPSE的高效液相色谱图和薄层色谱显示存在黄酮类化合物。用GPE或GPSE（均为800 - 1000 μg/mL）预处理HepG2细胞可显著（P < 0.0001）保护细胞免受四氯化碳诱导的细胞毒性。此外，用两种提取物（均为1000 μg/mL）处理的细胞显示出正常形态（一般形态和细胞核形态），与仅暴露于四氯化碳的细胞中的凋亡特征形成对比。在这些实验中，GPSE也比GPE更有效。此外，四氯化碳导致天冬氨酸转氨酶（AST）（P < 0.05）和丙氨酸转氨酶（ALT）（P < 0.0001）水平显著升高，而GPE或GPSE显著（P < 0.0001）降低了这些水平，达到对照组中的值。总之，结果表明[水果名称未给出]果实对HepG2细胞免受四氯化碳诱导的毒性具有肝保护作用，这可能至少部分与抗氧化化合物尤其是黄酮类化合物的存在有关。
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