Department of Plant Production & Technologies, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Omer Halisdemir University, Niğde, Turkey.
Department of Plant Production & Technologies, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Omer Halisdemir University, Niğde, Turkey.
Chemosphere. 2020 Feb;240:124857. doi: 10.1016/j.chemosphere.2019.124857. Epub 2019 Sep 16.
Colorado potato beetle, Leptinotarsa decemlineata Say (coleoptera: chrysomelidae), is the important pest of potato all over the world. This insect pest is resistant to more than 50 active compounds belonging to various chemical groups. Potential of RNA interference (RNAi) was explored to knock down transcript levels of imidacloprid resistant genes in Colorado potato beetle (CPB) under laboratory conditions. Three important genes belonging to cuticular protein (CP), cytochrome P450 monoxygenases (P450) and glutathione synthetase (GSS) families encoding imidacloprid resistance were targeted. Feeding bio-assays were conducted on various stages of imidacloprid resistant CPB lab population by applying HT115 expressing dsRNA on potato leaflets. Survival rate of insects exposed to CP-dsRNA decreased to 4.23%, 15.32% and 47.35% in 2nd, 3rd and 4th instar larvae respectively. Larval weight and pre-adult duration were also affected due to dsRNAs feeding. Synergism of RNAi with imidacloprid conducted on the 2nd instar larvae, exhibited 100% mortality of larvae when subjected to reduced doses of GSS and CP dsRNAs along with imidacloprid. Utilization of three different dsRNAs against imidacloprid resistant CPB population reveal that dsRNAs targeting CP, P450 and GSS enzymes could be useful tool in management of imidacloprid resistant CPB populations.
科罗拉多马铃薯甲虫，Leptinotarsa decemlineata Say（鞘翅目：叶甲科），是全世界马铃薯的重要害虫。这种昆虫对属于各种化学基团的 50 多种活性化合物具有抗性。在实验室条件下，探索了 RNA 干扰（RNAi）的潜力，以敲低科罗拉多马铃薯甲虫（CPB）中抗吡虫啉的基因的转录水平。针对编码抗吡虫啉的几丁质蛋白（CP）、细胞色素 P450 单加氧酶（P450）和谷胱甘肽合成酶（GSS）家族的 3 个重要基因进行了研究。通过在马铃薯叶片上应用表达 dsRNA 的 HT115，对具有抗吡虫啉特性的 CPB 实验室种群的各个阶段进行了喂食生物测定。暴露于 CP-dsRNA 的昆虫存活率在 2 龄、3 龄和 4 龄幼虫中分别下降到 4.23%、15.32%和 47.35%。dsRNA 喂食还影响了幼虫的体重和预成虫期。在 2 龄幼虫上进行的 RNAi 与吡虫啉的协同作用，当用吡虫啉与 GSS 和 CP dsRNA 的低剂量处理时，表现出 100%的幼虫死亡率。针对抗吡虫啉的 CPB 种群的三种不同 dsRNA 的利用表明，针对 CP、P450 和 GSS 酶的 dsRNA 可能是管理抗吡虫啉 CPB 种群的有用工具。
