Pathak Jyoti, Ramasamy Gandhi Gracy, Agrawal Aditi, Srivastava Subhi, Basavaarya Bhusangar Raghavendra, Muthugounder Mohan, Muniyappa Venugopal Kundalagurki, Maria Pratheepa, Rai Anil, Venkatesan Thiruvengadam
Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, P. Bag No. 2491, H.A. Farm Post Bellary Road, Hebbal, Bangalore 560024, India.
Centre for Agricultural Bioinformatics, Indian Agricultural Statistical Research Institute, Pusa, New Delhi 110012, India.
Insects. 2022 Oct 3;13(10):900. doi: 10.3390/insects13100900.
The aphid lion, (Neuroptera: Chrysopidae) is a highly effective beneficial predator of many agricultural pests and has developed resistance to several insecticides. Understanding the molecular mechanism of insecticide resistance in the predators is crucial for its effective application in IPM programs. Therefore, transcriptomes of imidacloprid-resistant and susceptible strains have been assessed using RNA-seq. Cytochrome P450 is one of the important gene families involved in xenobiotic metabolism. Hence, our study focused on the CYP gene family where mining, nomenclature, and phylogenetic analysis revealed a total of 95 unique CYP genes with considerable expansion in CYP3 and CYP4 clans. Further, differential gene expression (DGE) analysis revealed ten CYP genes from CYP3 and CYP4 clans to be differentially expressed, out of which nine genes (CYP4419A1, CYP4XK1, CYP4416A10, CYP4416A-fragment8, CYP6YL1, CYP6YH6, CYP9GK-fragment16, CYP9GN2, CYP9GK6) were downregulated and one (CYP9GK3) was upregulated in the resistant strain as compared to the susceptible strain. Expression validation by quantitative real-time PCR (qRT-PCR) is consistent with the DGE results. The expansion and differential expression of CYP genes may be an indicator of the capacity of the predator to detoxify a particular group of insecticides.
蚜狮（脉翅目：草蛉科）是许多农业害虫的高效有益捕食者，并且已对几种杀虫剂产生抗性。了解捕食者中杀虫剂抗性的分子机制对于其在综合害虫管理计划中的有效应用至关重要。因此，已使用RNA测序评估了吡虫啉抗性和敏感品系的转录组。细胞色素P450是参与异源生物代谢的重要基因家族之一。因此，我们的研究聚焦于CYP基因家族，其中的挖掘、命名和系统发育分析揭示了总共95个独特的CYP基因，在CYP3和CYP4家族中有显著扩增。此外，差异基因表达（DGE）分析显示，来自CYP3和CYP4家族的10个CYP基因存在差异表达，其中9个基因（CYP4419A^1、CYP4XK^1、CYP4416A^10、CYP4416A-片段8、CYP6YL^1、CYP6YH^6、CYP9GK-片段16、CYP9GN^2、CYP9GK^6）在抗性品系中相对于敏感品系下调，1个基因（CYP9GK^3）上调。通过定量实时PCR（qRT-PCR）进行的表达验证与DGE结果一致。CYP基因的扩增和差异表达可能是捕食者对特定杀虫剂解毒能力的一个指标。
