用于通过氙气超极化化学交换饱和转移核磁共振检测蛋白酶的轮烷探针。
Rotaxane probes for protease detection by Xe hyperCEST NMR.
作者信息
Slack Clancy C, Finbloom Joel A, Jeong Keunhong, Bruns Carson J, Wemmer David E, Pines Alexander, Francis Matthew B
机构信息
Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
出版信息
Chem Commun (Camb). 2017 Jan 17;53(6):1076-1079. doi: 10.1039/c6cc09302g.
We report a CB6 rotaxane for the Xe hyperCEST NMR detection of matrix metalloprotease 2 (MMP-2) activity. MMP-2 is overexpressed in cancer tissue, and hence is a cancer marker. A peptide containing an MMP-2 recognition sequence was incorporated into the rotaxane, synthesized via CB6-promoted click chemistry. Upon cleavage of the rotaxane by MMP-2, CB6 became accessible for Xe@CB6 interactions, leading to protease-responsive hyperCEST activation.
我们报道了一种用于通过氙气（Xe）的化学交换饱和转移（hyperCEST）核磁共振检测基质金属蛋白酶2（MMP-2）活性的CB6轮烷。MMP-2在癌组织中过表达，因此是一种癌症标志物。通过CB6促进的点击化学合成的轮烷中掺入了含有MMP-2识别序列的肽。当MMP-2切割轮烷时，CB6可用于Xe@CB6相互作用，从而导致蛋白酶响应性的hyperCEST激活。
Zotero 插件