AbdelRahim Khalid, Mahmoud Sabry Younis, Ali Ahmed Mohamed, Almaary Khalid Salmeen, Mustafa Abd El-Zaher M A, Husseiny Sherif Moussa
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Botany Department, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Department of Medical Laboratory Technology, College of Applied Medical Science, University of Dammam, 1704, Hafr Al Batin 319 91, Saudi Arabia.
Saudi J Biol Sci. 2017 Jan;24(1):208-216. doi: 10.1016/j.sjbs.2016.02.025. Epub 2016 Mar 10.
Synthesis of silver nanoparticles (AgNPs) has become a necessary field of applied science. Biological method for synthesis of AgNPs by aqueous mycelial extract was used. The AgNPs were identified by UV-visible spectrometry, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FT-IR). The presence of surface plasmon band around 420 nm indicates AgNPs formation. The characteristic of the AgNPs within the face-centered cubic (fcc) structure are indicated by the peaks of the X-ray diffraction (XRD) pattern corresponding to (1 1 1), (2 0 0) and (2 2 0) planes. Spherical, mono-dispersed and stable AgNPs with diameter around 9.47 nm were prepared and affirmed by high-resolution transmission electron microscopy (HR-TEM). Fourier Transform Infrared (FTIR) shows peaks at 1426 and 1684 cm that affirm the presence of coat covering protein the AgNPs which is known as capping proteins. Parameter optimization showed the smallest size of AgNPs (2.86 ± 0.3 nm) was obtained with 10 M AgNO at 40 °C. The present study provides the proof that the molecules within aqueous mycelial extract of facilitate synthesis of AgNPs and highlight on value-added from for cost effectiveness. Also, eco-friendly medical and nanotechnology-based industries could also be provided. Size of prepared AgNPs could be controlled by temperature and AgNO concentration. Further studies are required to study effect of more parameters on size and morphology of AgNPs as this will help in the control of large scale production of biogenic AgNPs.
银纳米颗粒（AgNPs）的合成已成为应用科学的一个必要领域。采用了利用水性菌丝体提取物合成AgNPs的生物学方法。通过紫外可见光谱、X射线衍射（XRD）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FT-IR）对AgNPs进行了鉴定。420nm左右表面等离子体带的存在表明AgNPs的形成。X射线衍射（XRD）图谱中对应于（1 1 1）、（2 0 0）和（2 2 0）平面的峰表明了面心立方（fcc）结构内AgNPs的特征。制备了直径约为9.47nm的球形、单分散且稳定的AgNPs，并通过高分辨率透射电子显微镜（HR-TEM）进行了确认。傅里叶变换红外光谱（FTIR）在1426和1684cm处出现峰，证实了覆盖在AgNPs上的蛋白质涂层（即封端蛋白）的存在。参数优化表明，在40℃下使用10M硝酸银可获得最小尺寸的AgNPs（2.86±0.3nm）。本研究证明了水性菌丝体提取物中的分子促进了AgNPs的合成，并突出了其在成本效益方面的附加值。此外，还可为环保型医疗和纳米技术产业提供支持。制备的AgNPs的尺寸可通过温度和硝酸银浓度进行控制。需要进一步研究更多参数对AgNPs尺寸和形态的影响，因为这将有助于控制生物源AgNPs的大规模生产。
