Department of NanoEngineering and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.
Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):10980-5. doi: 10.1073/pnas.1106634108. Epub 2011 Jun 20.
Efforts to extend nanoparticle residence time in vivo have inspired many strategies in particle surface modifications to bypass macrophage uptake and systemic clearance. Here we report a top-down biomimetic approach in particle functionalization by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery. The structure, size and surface zeta potential, and protein contents of the erythrocyte membrane-coated nanoparticles were verified using transmission electron microscopy, dynamic light scattering, and gel electrophoresis, respectively. Mice injections with fluorophore-loaded nanoparticles revealed superior circulation half-life by the erythrocyte-mimicking nanoparticles as compared to control particles coated with the state-of-the-art synthetic stealth materials. Biodistribution study revealed significant particle retention in the blood 72 h following the particle injection. The translocation of natural cellular membranes, their associated proteins, and the corresponding functionalities to the surface of synthetic particles represents a unique approach in nanoparticle functionalization.
为了延长纳米颗粒在体内的停留时间，人们在颗粒表面修饰方面进行了许多研究，以避免巨噬细胞摄取和全身清除。在这里，我们报告了一种自上而下的仿生方法，通过用天然红细胞膜（包括膜脂质和相关的膜蛋白）对可生物降解的聚合物纳米颗粒进行涂层，实现长循环货物输送。使用透射电子显微镜、动态光散射和凝胶电泳分别验证了红细胞膜涂层纳米颗粒的结构、尺寸、表面 zeta 电位和蛋白质含量。与用最先进的合成隐形材料涂层的对照颗粒相比，用红细胞模拟纳米颗粒进行小鼠注射后，荧光染料负载的纳米颗粒的循环半衰期明显延长。在粒子注射后 72 小时的体内分布研究中发现了显著的粒子保留。将天然细胞膜及其相关蛋白和相应功能转移到合成颗粒表面是一种独特的纳米颗粒功能化方法。
