Yang Ming-Hui, Chen Ko-Chin, Chiang Pei-Wen, Chung Tze-Wen, Chen Wan-Jou, Chu Pei-Yu, Chen Sharon Chia-Ju, Lu Yi-Shan, Yuan Cheng-Hui, Wang Ming-Chen, Lin Chia-Yang, Huang Ying-Fong, Jong Shiang-Bin, Lin Po-Chiao, Tyan Yu-Chang
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan.
Biomed Res Int. 2016;2016:1917394. doi: 10.1155/2016/1917394. Epub 2016 Dec 7.
The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid (HA) biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media (PC12 CM) in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS/MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.
神经元细胞的微环境在调节神经发育和再生中起着至关重要的作用。透明质酸（HA）生物材料已被广泛应用于众多医学和生物学领域，并在神经再生中发挥重要作用。据报道，PC12细胞能够内源性合成和分泌神经生长因子（NGF）。本研究的目的是评估HA生物材料与PC12细胞条件培养基（PC12 CM）联合应用在神经再生中的作用。以SH-SY5Y细胞作为实验模型，我们发现用PC12 CM进行支持可增强HA在SH-SY5Y细胞增殖和黏附中的功能。通过反相纳米超高效液相色谱-电喷雾串联质谱（RP-nano-UPLC-ESI-MS/MS）分析，我们鉴定出在HA修饰表面上与PC12 CM共同处理培养的SH-SY5Y细胞中，热休克蛋白60（HSP60）和Ran结合蛋白2（RanBP2）的表达增加。此外，我们还鉴定出了PC12细胞分泌的、可能促进SH-SY5Y细胞增殖和黏附的因子。在此，我们提出了一种富含神经营养因子的生物材料表面，用于神经再生应用。
