Huang Yanping, Gao Shixing, Chen Jinglong, Albrecht Elke, Zhao Ruqian, Yang Xiaojing
Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Leibniz Institute for Farm Animal Biology, Institute for Muscle Biology and Growth, Dummerstorf, Germany.
Oncotarget. 2017 Feb 21;8(8):13073-13084. doi: 10.18632/oncotarget.14375.
Maternal nutrition is important for the risk of the offspring to develop insulin resistance and adiposity later in life. The study was undertaken to determine effects of maternal butyrate supplementation on lipid metabolism and insulin sensitivity in the offspring skeletal muscle. The offspring of rats, fed a control diet or a butyrate diet (1% sodium butyrate) throughout gestation and lactation, was studied at weaning and at 60 days of age. The offspring of dams fed a butyrate diet had higher HOMA-insulin resistance and impaired glucose tolerance. This was associated with elevated mRNA and protein expressions of lipogenic genes and decreased amounts of lipolytic enzyme. Simultaneously, enhanced acetylation of histone H3 lysine 9 and histone H3 lysine 27 modification on the lipogenic genes in skeletal muscle of adult offspring was observed. Higher concentration of serum insulin and intramuscular triglyceride in skeletal muscle of offspring from the butyrate group at weaning were accompanied by increasing levels of lipogenic genes and enrichment of acetylation of histone H3 lysine 27. Maternal butyrate supplementation leads to insulin resistance and ectopic lipid accumulation in skeletal muscle of offspring, indicating the importance of short chain fatty acids in the maternal diet on lipid metabolism.
母体营养对于后代日后发生胰岛素抵抗和肥胖的风险至关重要。本研究旨在确定母体补充丁酸盐对后代骨骼肌脂质代谢和胰岛素敏感性的影响。在整个妊娠和哺乳期喂食对照饮食或丁酸盐饮食（1% 丁酸钠）的大鼠后代，在断奶时和 60 日龄时进行了研究。喂食丁酸盐饮食的母鼠后代具有更高的HOMA-胰岛素抵抗和受损的糖耐量。这与脂肪生成基因的mRNA和蛋白质表达升高以及脂解酶数量减少有关。同时，在成年后代的骨骼肌中观察到脂肪生成基因上组蛋白H3赖氨酸9的乙酰化增强和组蛋白H3赖氨酸27修饰。断奶时丁酸盐组后代骨骼肌中较高的血清胰岛素浓度和肌内甘油三酯伴随着脂肪生成基因水平的增加和组蛋白H3赖氨酸27乙酰化的富集。母体补充丁酸盐会导致后代骨骼肌出现胰岛素抵抗和异位脂质积累，表明母体饮食中短链脂肪酸对脂质代谢的重要性。
