Kandolf R, Kirschner P, Hofschneider P H, Vischer T L
Department of Virus Research, Max-Planck-Institute for Biochemistry, Martinsried, Federal Republic of Germany.
Clin Rheumatol. 1989 Sep;8(3):398-401. doi: 10.1007/BF02030355.
We used in situ hybridization to search for the presence of viruses in synovial fluid cell preparations obtained from patients with various forms of knee arthropathies. One patient, presenting with acute reactive arthritis, was found to replicate parvovirus DNA in cells from synovial fluid whereas six other patients with various other forms of arthritis were negative for parvovirus infection. Five patients with osteoarthritis, constituting a control group in which an infectious etiology would not be expected, were consistently negative. In addition, no hybridization was found when synovial cell preparations of all patients were hybridized in situ with DNA probes specific for Epstein-Barr virus, cytomegalovirus, or enteroviruses.
我们使用原位杂交技术，在从患有各种形式膝关节病的患者中获取的滑液细胞制剂中寻找病毒的存在。一名表现为急性反应性关节炎的患者被发现其滑液中的细胞能复制细小病毒DNA，而其他六名患有各种其他形式关节炎的患者细小病毒感染呈阴性。五名骨关节炎患者作为一个对照组（在此组中不预期有感染性病因），结果始终为阴性。此外，当所有患者的滑膜细胞制剂与针对爱泼斯坦-巴尔病毒、巨细胞病毒或肠道病毒的DNA探针进行原位杂交时，未发现杂交现象。
