Wei Yanqiu, Yang Baozhi, Li Yunlong, Duan Yongcheng, Tian Deyu, He Baoxiang, Chen Chuangfu, Liu Wenjun, Yang Limin
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning 530004, China.
J Vet Sci. 2020 Jul;21(4):e68. doi: 10.4142/jvs.2020.21.e68.
A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.
开发了一种基于荧光微球的免疫层析试纸条检测法（FICT），用于在猪舍现场快速、灵敏且定量地检测猪繁殖与呼吸综合征病毒（PRRSV）抗体。该检测法基于夹心免疫复合物（抗猪IgG-PRRSV抗体-NSP7/N）的形成，通过与使用3325份临床样本的IDEXX-ELISA进行比较来验证。FICT的诊断特异性、敏感性和准确性分别为97.28%、93.41%和94.95%。FICT与病毒中和试验显示出良好的相关性。总体而言，开发了一种有前景的猪舍现场诊断工具，可在15分钟内快速定量检测PRRSV抗体。
