Imori Priscilla F M, Passaglia Jaqueline, Souza Roberto A, Rocha Lenaldo B, Falcão Juliana P
Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirao Preto, Universidade de Sao Paulo - USP, Ribeirao Preto, SP, 14040-903, Brazil.
Departamento de Patologia, Genética e Evolução, Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, MG, 38040-500, Brazil; Departamento de Patologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo - USP, Ribeirão Preto, SP, 14049-900, Brazil.
Microb Pathog. 2017 Mar;104:72-77. doi: 10.1016/j.micpath.2017.01.008. Epub 2017 Jan 3.
Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cells. A total of 50 Y. frederiksenii, 55 Y. intermedia and 13 Y. kristensenii strains were studied. The strains contained the following genes: Y. frederiksenii, fepA(44%), fes(44%) and ystB(18%); Y. intermedia, ail(53%), fepA (35%), fepD(2%), fes(97%), hreP(2%), ystB(2%) and tccC(35%); Y. kristensenii, ail(62%), ystB(23%), fepA(77%), fepD(54%), fes(54%) and hreP(77%). Generally, the Y. enterocolitica-like strains had a reduced ability to adhere to and invade mammalian cells compared to the highly pathogenic Y. enterocolitica 8081. However, Y. kristensenii FCF410 and Y. frederiksenii FCF461 presented high invasion potentials in Caco-2 cells after five days of pre-incubation increased by 45- and 7.2-fold compared to Y. enterocolitica 8081, respectively; but, the ail gene was not detected in these strains. The presence of virulence-related genes in some of the Y. enterocolitica-like strains indicated their possible pathogenic potential. Moreover, the results suggest the existence of alternative virulence mechanisms and that the pathogenicity of Y. kristensenii and Y. frederiksenii may be strain-dependent.
类小肠结肠炎耶尔森菌在致病潜力方面尚未得到广泛研究。这项工作旨在通过PCR评估一些类小肠结肠炎耶尔森菌菌株中与毒力相关基因的存在情况，以及它们黏附并侵袭Caco-2和HEp-2细胞的能力，从而评估这些菌株的致病潜力。共研究了50株费氏耶尔森菌、55株中间耶尔森菌和13株克里斯滕森耶尔森菌。这些菌株含有以下基因：费氏耶尔森菌，fepA（44%）、fes（44%）和ystB（18%）；中间耶尔森菌，ail（53%）、fepA（35%）、fepD（2%）、fes（97%）、hreP（2%）、ystB（2%）和tccC（35%）；克里斯滕森耶尔森菌，ail（62%）、ystB（23%）、fepA（77%）、fepD（54%）、fes（54%）和hreP（77%）。一般来说，与高致病性的小肠结肠炎耶尔森菌8081相比，类小肠结肠炎耶尔森菌菌株黏附并侵袭哺乳动物细胞的能力有所降低。然而，克里斯滕森耶尔森菌FCF410和费氏耶尔森菌FCF461在预孵育5天后，在Caco-2细胞中表现出较高的侵袭潜力，分别比小肠结肠炎耶尔森菌8081增加了45倍和7.2倍；但是，在这些菌株中未检测到ail基因。一些类小肠结肠炎耶尔森菌菌株中与毒力相关基因的存在表明它们可能具有致病潜力。此外，结果表明存在替代毒力机制，并且克里斯滕森耶尔森菌和费氏耶尔森菌的致病性可能因菌株而异。
