OMFS IMPATH Research Group, Department of Imaging & Pathology, Faculty of Medicine, KU Leuven, Kapucijnenvoer 33, 3000, Leuven, Belgium.
Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium.
Int J Implant Dent. 2021 Feb 18;7(1):11. doi: 10.1186/s40729-021-00291-5.
Bone-to-implant contact ratio (BIC%) plays a critical role in secondary stability of osseointegrated dental implants. The aim of this study was to identify the correlation of 2D/3D micro-CT images with histology as a gold standard for evaluating BIC% and to investigate the influence of the platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on 3D BIC% following delayed implant placement with delayed loading (DIP+DL).
Nine beagle dogs were recruited. Following bilateral extraction of mandibular 3rd premolar, 4th premolar, and 1st molar, 54 screw-type titanium implants were inserted and randomly divided into one control and two test groups based on a split-mouth design. The control group involved DIP+DL (n = 18) and both test groups included DIP+DL with local application of PRP (n = 18) and PPP (n = 18). A BIC analysis was performed utilizing 2D histomorphometry and 2D/3D micro-CT. Following identification of correlation between histology and 2D/3D micro-CT images, a 3D micro-CT assessment of the 3D BIC% at three follow-up time-points (1, 3, and 6 months) was carried out for observing the influence of PRP and PPP on BIC.
The 2D micro-CT BIC% values revealed a strong positive correlation with histology (r = 0.98, p < 0.001) and a moderate correlation existed with 3D micro-CT (r = 0. 67, p = 0.005). BIC levels at 1 month and combined influence of PPP and PRP irrespective of time-points revealed significantly higher 3D BIC% compared to the control. However, a reduction in 3D BIC% was observed at the 3rd and 6th month. No significant difference was observed between both PRP and PPP.
Both 2D and 3D micro-CT demonstrated a potential to be utilized as a complimentary method for assessing BIC compared to the histological gold standard. Overall, both PRP and PPP significantly facilitated bone healing and osseointegration with a higher 3D BIC at follow-up. However, their influence was reduced as the observation period was increased.
骨-种植体接触比（BIC%）在骨整合种植体的二次稳定性中起着关键作用。本研究的目的是确定 2D/3D 微 CT 图像与组织学的相关性，以评估 BIC%作为金标准，并研究富血小板血浆（PRP）和贫血小板血浆（PPP）对延迟植入和延迟加载（DIP+DL）后 3D BIC%的影响。
招募了 9 只比格犬。在双侧下颌第三前磨牙、第四前磨牙和第一磨牙拔除后，插入 54 个螺丝式钛种植体，并根据分割口腔设计随机分为对照组和两组实验组。对照组为 DIP+DL（n=18），两组实验组分别为 DIP+DL 局部应用 PRP（n=18）和 PPP（n=18）。利用 2D 组织形态计量学和 2D/3D 微 CT 进行 BIC 分析。在确定组织学与 2D/3D 微 CT 图像之间的相关性后，在三个随访时间点（1、3 和 6 个月）进行 3D 微 CT 评估，观察 PRP 和 PPP 对 BIC 的影响。
2D 微 CT BIC%值与组织学呈强正相关（r=0.98，p<0.001），与 3D 微 CT 呈中度相关（r=0.67，p=0.005）。1 个月时的 BIC 水平以及 PPP 和 PRP 的综合影响，无论时间点如何，均显示出明显更高的 3D BIC%，与对照组相比。然而，在第 3 个月和第 6 个月观察到 3D BIC%降低。PRP 和 PPP 之间无显著差异。
2D 和 3D 微 CT 均显示出与组织学金标准相比，作为评估 BIC 的补充方法具有潜力。总体而言，PRP 和 PPP 均显著促进骨愈合和骨整合，在随访时具有更高的 3D BIC%。然而，随着观察期的延长，它们的影响会降低。
