载脂蛋白 CETP Taq1B 多态性与饮食脂肪摄入对 2 型糖尿病血脂正常和血脂异常患者脂谱的交互作用差异。

Differences in the interaction between CETP Taq1B polymorphism and dietary fat intake on lipid profile of normolipedemic and dyslipidemic patients with type 2 diabetes mellitus.

机构信息

Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.

Department of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Clin Nutr. 2018 Feb;37(1):270-275. doi: 10.1016/j.clnu.2016.12.024. Epub 2016 Dec 31.

DOI:10.1016/j.clnu.2016.12.024

PMID:28065481

Abstract

BACKGROUND & AIM: Dyslipidemia is one of the major complications in patients with type 2 diabetes mellitus (T2DM). Dietary fat intake and genetic factors including CETP Taq1B polymorphism could also affect lipid profile concentrations, in particular HDL-c. We decided to study the frequency of this polymorphism and its interaction with dietary fat intake on HDL-c concentration among Iranian T2DM patients with and without dyslipidemia.

METHODS

In this comparative study, serum samples were collected from 55 patients with dyslipidemia and 129 patients without dyslipidemia. Validated semi-quantitative FFQ was used for food consumption data. CETP Taq1B polymorphism was studied by polymerase chain reaction-restriction length polymorphism (PCR-RFLP). We used χ and two-way ANOVA tests for statistical analysis.

RESULTS

The frequency of B1B1 genotype was higher in patients with dyslipidemia (p = 0.01). There was no significant relationship between CETP Taq1B polymorphism and lipid profile concentrations. In patients without dyslipidemia, the interaction between the polymorphism and total fat intake on HDL-c concentration as well as TG/HDL ratio was significant (p = 0.02 and p = 0.009 respectively). This was more evident in B1B1 genotype. Moreover, HDL-c concentration was significantly higher in B2B2 genotype with low total fat intake.

CONCLUSION

Higher total fat intake may affect the relationship between CETP Taq1B polymorphism and HDL-c concentration in patients with normolipidemic T2DM.

摘要

背景与目的

血脂异常是 2 型糖尿病（T2DM）患者的主要并发症之一。膳食脂肪摄入和包括 CETP Taq1B 多态性在内的遗传因素也可能影响血脂谱浓度，尤其是 HDL-c。我们决定研究该多态性的频率及其与伊朗 T2DM 患者血脂异常和无血脂异常患者的饮食脂肪摄入对 HDL-c 浓度的相互作用。

方法

在这项比较研究中，从 55 名血脂异常患者和 129 名无血脂异常患者中采集血清样本。使用经过验证的半定量 FFQ 收集食物消费数据。通过聚合酶链反应-限制性长度多态性（PCR-RFLP）研究 CETP Taq1B 多态性。我们使用 χ 和双向 ANOVA 检验进行统计分析。

结果

血脂异常患者 B1B1 基因型的频率更高（p=0.01）。CETP Taq1B 多态性与血脂谱浓度之间没有显著关系。在无血脂异常的患者中，多态性与总脂肪摄入量对 HDL-c 浓度以及 TG/HDL 比值的相互作用具有显著性（分别为 p=0.02 和 p=0.009）。在 B1B1 基因型中更为明显。此外，B2B2 基因型的总脂肪摄入量低时，HDL-c 浓度显著升高。