Zhang Yi, Ruan Jingya, Li Jian, Chao Liping, Shi Wenzhong, Yu Haiyang, Gao Xiumei, Wang Tao
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China; Tianjin Key Laboratory of TCM Chemistry and Analysis, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China.
Fitoterapia. 2017 Mar;117:28-33. doi: 10.1016/j.fitote.2017.01.004. Epub 2017 Jan 5.
The 70% ethanol extract of Dioscorea septemloba rhizomes was subjected to chromatographic columns including silica gel, ODS, D101 resin, Sephadex LH-20 and HPLC, to yield three new diarylheptanoids (1-3) and two novel stilbenes (4 and 5), together with six known ones (6-11). Their structures were elucidated on the basis of extensive 1D NMR (H and C), 2D NMR (COSY, HSQC, HMBC, NOESY), IR, and MS spectroscopic data analyses. Moreover, the in vitro bioactivity screening experiment suggested that compounds 6-8 and 10 could significantly increase the glucose consumption in differentiated L6 myotubes, and that 2, 9-11 displayed triglyceride inhibitory effects in HepG2 cells.
对绵萆薢根茎的70%乙醇提取物进行硅胶柱、ODS柱、D101树脂柱、葡聚糖凝胶LH - 20柱和高效液相色谱分离，得到3个新的二芳基庚烷类化合物（1 - 3）和2个新的芪类化合物（4和5），以及6个已知化合物（6 - 11）。通过广泛的一维核磁共振（氢谱和碳谱）、二维核磁共振（COSY、HSQC、HMBC、NOESY）、红外光谱和质谱数据分析阐明了它们的结构。此外，体外生物活性筛选实验表明，化合物6 - 8和10可显著增加分化的L6肌管中的葡萄糖消耗，而化合物2、9 - 11对HepG2细胞具有甘油三酯抑制作用。
