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非双层结构在调节线粒体膜中ATP合酶活性方面的潜在作用。

The Possible Role of Nonbilayer Structures in Regulating ATP Synthase Activity in Mitochondrial Membranes.

作者信息

Gasanov S E, Kim A A, Dagda R K

机构信息

Moscow State University Branch, 22a A. Timur Avenue, Tashkent, 100060 Uzbekistan.

Department of Pharmacology, University of Nevada, Reno School of Medicine,1664 North Virginia St., Reno, Nevada, 89557 United States.

出版信息

Biophysics (Oxf). 2016 Jul;61(4):596-600. doi: 10.1134/S0006350916040084. Epub 2016 Oct 19.

DOI:10.1134/S0006350916040084
PMID:28065984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5215001/
Abstract

The effects of temperature and of the membrane-active protein CTII on the formation of nonbilayer structures in mitochondrial membranes were studied by P-NMR. Increasing the temperature of isolated mitochondrial fractions correlated with an increase in ATP synthase activity and the formation of nonbilayer packed phospholipids with immobilized molecular mobility. Computer modeling was employed for analyzing the interaction of mitochondrial membrane phospholipids with the molecular surface of CTII, which behaves like a dicyclohexylcarbodiimide-binding protein (DCCD-BP) of the F group in a lipid phase. Overall, our studies suggest that proton permeability toroidal pores formed in mitochondrial membranes consist of immobilized nonbilayer-packed phospholipids formed via interactions with DCCD-BP. Our studies support the existence of a proton transport along a concentration gradient mediated via transit toroidal permeability pores which induce conformational changes necessary for mediating the catalytic activity of ATP synthase in the subunits of the F-F complex.

摘要

通过磷核磁共振研究了温度和膜活性蛋白CTII对线粒体膜中非双层结构形成的影响。提高分离的线粒体组分的温度与ATP合酶活性的增加以及具有固定分子流动性的非双层堆积磷脂的形成相关。采用计算机建模分析线粒体膜磷脂与CTII分子表面的相互作用，CTII在脂质相中表现得像F组的二环己基碳二亚胺结合蛋白（DCCD-BP）。总体而言，我们的研究表明，线粒体膜中形成的质子渗透性环形孔由通过与DCCD-BP相互作用形成的固定非双层堆积磷脂组成。我们的研究支持通过转运环形渗透孔介导沿浓度梯度的质子转运的存在，这些孔诱导F-F复合体亚基中ATP合酶催化活性所需的构象变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/60d69beb8f8f/nihms839101f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/18e6d228d98a/nihms839101f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/e0e6c63046f7/nihms839101f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/60d69beb8f8f/nihms839101f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/18e6d228d98a/nihms839101f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/e0e6c63046f7/nihms839101f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6915/5215001/60d69beb8f8f/nihms839101f3.jpg

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