Gasanov S E, Vernon L P, Aripov T F
Department of Biological Sciences, University of Texas, El Paso 79968-0519.
Arch Biochem Biophys. 1993 Mar;301(2):367-74. doi: 10.1006/abbi.1993.1157.
Pyrularia thionin (P thionin) is a bioactive peptide from the parasitic plant Pyrularia pubera. The structural aspects of its interaction with phospholipid membranes were investigated by measuring the responses of phosphorescence quenching, EPR spin labels, and 1H and 31P NMR at different phospholipid compositions. In phosphatidylcholine bilayers containing cardiolipin or phosphatidylinositol, P thionin induced a pronounced increase in the membrane viscosity, and at higher P thionin concentrations the formation of nonbilayer structures was observed. In phosphatidylcholine bilayers containing phosphatidylserine, P thionin induced a significant transformation of membrane lamellar structure accompanied by a decrease of membrane viscosity. In all investigated lipid systems added P thionin caused an increase in membrane permeability and induced a fusion of sonicated liposomes. The specificity of P thionin interaction with phosphatidylserine-containing membrane is discussed and a model of phospholipid membrane modification by P thionin is suggested.
野菰硫堇（P thionin）是一种来自寄生植物野菰的生物活性肽。通过测量不同磷脂组成下的磷光猝灭、电子顺磁共振自旋标记以及1H和31P核磁共振响应，研究了其与磷脂膜相互作用的结构方面。在含有心磷脂或磷脂酰肌醇的磷脂酰胆碱双层中，P thionin导致膜粘度显著增加，并且在较高的P thionin浓度下观察到非双层结构的形成。在含有磷脂酰丝氨酸的磷脂酰胆碱双层中，P thionin导致膜层状结构发生显著转变，同时膜粘度降低。在所有研究的脂质体系中，添加的P thionin导致膜通透性增加并诱导超声处理的脂质体融合。讨论了P thionin与含磷脂酰丝氨酸膜相互作用的特异性，并提出了P thionin修饰磷脂膜的模型。
