Kwenda Stanford, Motlolometsi Tshepiso V, Birch Paul R J, Moleleki Lucy N
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, Genomics Research Institute, University of Pretoria Pretoria, South Africa.
Division of Plant Sciences, James Hutton Institute, College of Life Sciences, University of Dundee Dundee, UK.
Front Plant Sci. 2016 Dec 20;7:1905. doi: 10.3389/fpls.2016.01905. eCollection 2016.
subsp. is a member of the soft rot Enterobacteriaceae (SRE) family that causes tuber soft rot and blackleg diseases of stems in potato plants. Currently, there are no effective chemical strategies for the control of members of the SRE. Thus, an understanding of the inducible defense responses in stems of potato plants is important, particularly during colonization of the vascular system. Here, time-course RNA-sequencing analysis was used to compare expressed genes between a susceptible potato cultivar ( cv Valor) and a tolerant cultivar ( cv BP1) at 0, 6, 12, 24, and 72 h post-inoculation with . In total, we identified 6139 and 8214 differentially expressed genes (DEGs) in the tolerant and susceptible cultivars, compared to mock-inoculated controls, respectively. Key DEGs distinguishing between tolerance and susceptibility were associated with negative regulation of cell death and plant-type cell wall organization/biogenesis biological processes in the tolerant and susceptible cultivars, respectively. Among these were DEGs involved in signaling (mainly MAPK cascade and ethylene pathway), defense-related transcription regulation including WRKY transcription factors, and downstream secondary cell biosynthesis. Together, our results suggest that cv BP1 likely employs quantitative defense response against . Overall, our study provides the first transcriptome-wide insight into the molecular basis of tolerance and/or resistance of potato stems to SRE infection.
亚种是软腐肠杆菌科（SRE）家族的成员，可导致马铃薯植株块茎软腐和茎部黑胫病。目前，尚无有效的化学策略来控制SRE家族成员。因此，了解马铃薯植株茎中的诱导防御反应非常重要，尤其是在维管系统定殖期间。在此，采用时间进程RNA测序分析，比较了易感马铃薯品种（cv Valor）和耐性品种（cv BP1）在接种后0、6、12、24和72小时的基因表达情况。与模拟接种对照相比，我们总共在耐性和易感品种中分别鉴定出6139个和8214个差异表达基因（DEG）。区分耐性和易感性的关键DEG分别与耐性和易感品种中细胞死亡的负调控以及植物型细胞壁组织/生物合成生物学过程相关。其中包括参与信号传导（主要是MAPK级联和乙烯途径）、包括WRKY转录因子在内的防御相关转录调控以及下游次生细胞生物合成的DEG。总之，我们的结果表明cv BP1可能对采用定量防御反应。总体而言，我们的研究首次提供了全转录组范围的见解，以了解马铃薯茎对SRE感染的耐性和/或抗性的分子基础。
