College of Agriculture/College of Horticulture and Gardening/Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou 434025, China.
College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2017 Dec 27;19(1):71. doi: 10.3390/ijms19010071.
Little information about the roles of circular RNAs (circRNAs) during potato- subsp. () interaction is currently available. In this study, we conducted the systematic identification of circRNAs from time series samples of potato cultivars Valor (susceptible) and BP1 (disease tolerant) infected by . A total of 2098 circRNAs were detected and about half (931, 44.38%) were intergenic circRNAs. And differential expression analysis detected 429 significantly regulated circRNAs. circRNAs play roles by regulating parental genes and sponging miRNAs. Gene Ontology (GO) enrichment of parental genes and miRNAs targeted mRNAs revealed that these differentially expressed (DE) circRNAs were involved in defense response (GO:0006952), cell wall (GO:0005199), ADP binding (GO:0043531), phosphorylation (GO:0016310), and kinase activity (GO:0016301), suggesting the roles of circRNAs in regulating potato immune response. Furthermore, weighted gene co-expression network analysis (WGCNA) found that circRNAs were closely related with coding-genes and long intergenic noncoding RNAs (lincRNAs). And together they were cultivar-specifically regulated to strengthen immune response of potato to infection, implying the roles of circRNAs in reprogramming disease responsive transcriptome. Our results will provide new insights into the potato- interaction and may lead to novel disease control strategy in the future.
关于环状 RNA（circRNAs）在马铃薯亚种（）互作中的作用，目前的信息还很少。在这项研究中，我们对受感染的马铃薯品种 Valor（易感）和 BP1（抗病）的时间序列样本进行了环状 RNA 的系统鉴定。共检测到 2098 个 circRNAs，其中约一半（931 个，44.38%）为基因间 circRNAs。差异表达分析检测到 429 个显著调节的 circRNAs。circRNAs 通过调节母基因和海绵 miRNA 发挥作用。靶基因和 miRNA 靶向 mRNAs 的基因本体论（GO）富集揭示了这些差异表达的（DE）circRNAs参与防御反应（GO：0006952）、细胞壁（GO：0005199）、ADP 结合（GO：0043531）、磷酸化（GO：0016310）和激酶活性（GO：0016301），表明 circRNAs 在调节马铃薯免疫反应中的作用。此外，加权基因共表达网络分析（WGCNA）发现 circRNAs 与编码基因和长基因间非编码 RNA（lincRNAs）密切相关。它们共同受到品种特异性调节，以增强马铃薯对的免疫反应，暗示 circRNAs 在重新编程疾病响应转录组中的作用。我们的研究结果将为马铃薯-互作提供新的见解，并可能为未来的疾病控制策略提供新的思路。
