Tian Xiangrong, Li Sidi, Liu Yisong, Liu Xuanming
College of Biology, Hunan UniversityChangsha, China; Key Laboratory of Plant Resource Conservation and Utilization of Hunan Province, Jishou UniversityJishou, China.
Key Laboratory of Plant Resource Conservation and Utilization of Hunan Province, Jishou University Jishou, China.
Front Plant Sci. 2016 Dec 21;7:1921. doi: 10.3389/fpls.2016.01921. eCollection 2016.
Mungbean ( L. Wilczek) is an important legume crop for its valuable nutritional and health benefits. Desiccation tolerance (DT) is a capacity of seeds to survive and maintain physiological activities during storage and under stress conditions. Many studies of DT have been reported in other legume crop, such as soybean and with little studies in the mungbean. In this study, the transcript profiles of mungbean seeds under different imbibition times were investigated for DT using RNA-sequencing (RNA-seq). A total of 3210 differentially expressed genes (DEGs) were found at the key period of DT (3-18 h of imbibition). Gene ontology (GO) and KEGG analysis showed that the terms of "response to stimulus," "transcription regulator," "methylation," and "starch and sucrose metabolism" were enriched for DT. Clustering analysis also showed that many transcription factors (MYB, AP2, and NAC), HSPs, embryogenesis abundant (LEA) proteins, and genes encoding methyltransferase and histone were differentially expressed. Nine of these DEGs were further validated by quantitative RT-PCR (qRT-PCR). Our study extends our knowledge of mungbean transcriptomes and further provides insight into the molecular mechanism of DT as well as new strategies for developing drought-tolerant crops.
绿豆（Vigna radiata (L.) Wilczek）因其宝贵的营养和健康益处，是一种重要的豆类作物。脱水耐受性（DT）是种子在储存和胁迫条件下存活并维持生理活动的能力。在其他豆类作物如大豆中已经报道了许多关于脱水耐受性的研究，而在绿豆中的研究较少。在本研究中，利用RNA测序（RNA-seq）研究了不同吸胀时间下绿豆种子的转录谱以了解脱水耐受性。在脱水耐受性的关键时期（吸胀3 - 18小时）共发现3210个差异表达基因（DEG）。基因本体（GO）和KEGG分析表明，“对刺激的反应”、“转录调节因子”、“甲基化”以及“淀粉和蔗糖代谢”等术语在脱水耐受性方面显著富集。聚类分析还表明，许多转录因子（MYB、AP2和NAC）、热休克蛋白（HSP）、胚胎发育晚期丰富蛋白（LEA）以及编码甲基转移酶和组蛋白的基因差异表达。其中9个差异表达基因通过定量RT-PCR（qRT-PCR）进一步验证。我们的研究扩展了我们对绿豆转录组的认识，并进一步深入了解脱水耐受性的分子机制以及开发耐旱作物的新策略。
