Manukumar Honnayakanahalli Marichenne Gowda, Umesha Sharanaiah
Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Karnataka, India.
Acta Sci Pol Technol Aliment. 2015 Jan-Mar;14(1):85-90. doi: 10.17306/J.AFS.2015.1.10.
esent study was conducted to evaluate Manoflora (MF), Polyflora (PF), Polyflora forest (PFf), and Processed (Pro) honey varieties to compare the in-vitro anti-inflammatory effects of aqueous honey samples in dose dependent manner. In-vitro anti-inflammatory activity was evaluated using membrane stabilization assay of RBCs at different aqueous honey concentrations. Material and method. The present investigation carried out for selected varieties of honey against erythrocytes exposed to both heat and hypotonic lyses and inhibition of membrane damage was compared to the standard drug acetylsalicylic acid. Results. Membrane damage was inhibited in both the model hemolysis of erythrocytes in vitro in a concentration dependent manner. Hypotonic solution inducing damage was inhibited by aqueous honey sample in ascending order ranged from 8.25% to 97.76% at 10 to 50 mg/ml and standard drug acetylsalicylic acid showing hemolysis protection 96.09% at 100 μg/ml concentration. In heat induced hemolysis model aqueous honey sample exhibited its protecting property during external stress condition in all samples ranged from 0.44% to 21.23% at 10 to 50 mg/ml and acetylsalicylic acid showed 39.38% at 100 μg/ml concentration. Among the variety PFf showed highest protecting nature for hypotonic solution induced lyses (97.76%) and heat induced hemolysis (21.23%) at 50 mg/ml respectively. Conclusion. With these investigations data conclude that the model exhibits marked anti-inflammatory effect. Future research is to be carried out to identify the molecules responsible in honey and its mechanism.
present study was conducted to evaluate Manoflora (MF), Polyflora (PF), Polyflora forest (PFf), and Processed (Pro) honey varieties to compare the in-vitro anti-inflammatory effects of aqueous honey samples in dose dependent manner. In-vitro anti-inflammatory activity was evaluated using membrane stabilization assay of RBCs at different aqueous honey concentrations. Material and method. The present investigation carried out for selected varieties of honey against erythrocytes exposed to both heat and hypotonic lyses and inhibition of membrane damage was compared to the standard drug acetylsalicylic acid.
Membrane damage was inhibited in both the model hemolysis of erythrocytes in vitro in a concentration dependent manner. Hypotonic solution inducing damage was inhibited by aqueous honey sample in ascending order ranged from 8.25% to 97.76% at 10 to 50 mg/ml and standard drug acetylsalicylic acid showing hemolysis protection 96.09% at 100 μg/ml concentration. In heat induced hemolysis model aqueous honey sample exhibited its protecting property during external stress condition in all samples ranged from 0.44% to 21.23% at 10 to 50 mg/ml and acetylsalicylic acid showed 39.38% at 100 μg/ml concentration. Among the variety PFf showed highest protecting nature for hypotonic solution induced lyses (97.76%) and heat induced hemolysis (21.23%) at 50 mg/ml respectively.
With these investigations data conclude that the model exhibits marked anti-inflammatory effect. Future research is to be carried out to identify the molecules responsible in honey and its mechanism involved.
本研究旨在评估单花种蜂蜜（MF）、多花种蜂蜜（PF）、多花种森林蜂蜜（PFf）和加工蜂蜜（Pro）品种，以剂量依赖性方式比较蜂蜜水样品的体外抗炎作用。使用不同浓度蜂蜜水对红细胞进行膜稳定试验来评估体外抗炎活性。材料与方法。本研究针对选定的蜂蜜品种对暴露于热和低渗裂解的红细胞进行研究，并将膜损伤的抑制作用与标准药物乙酰水杨酸进行比较。结果。在体外红细胞模型溶血中，膜损伤均呈浓度依赖性受到抑制。低渗溶液诱导的损伤在10至50mg/ml时被蜂蜜水样品以升序抑制，抑制率从8.25%至97.76%，标准药物乙酰水杨酸在100μg/ml浓度时显示溶血保护率为96.09%。在热诱导溶血模型中，蜂蜜水样品在10至50mg/ml时，所有样品在外部应激条件下均表现出保护特性，保护率从0.44%至21.23%，乙酰水杨酸在100μg/ml浓度时显示保护率为39.38%。在这些品种中，PFf在50mg/ml时对低渗溶液诱导的裂解（97.76%）和热诱导的溶血（21.23%）表现出最高的保护特性。结论。根据这些研究数据得出结论，该模型具有显著的抗炎作用。未来需开展研究以确定蜂蜜中起作用的分子及其作用机制。
本研究旨在评估单花种蜂蜜（MF）、多花种蜂蜜（PF）、多花种森林蜂蜜（PFf）和加工蜂蜜（Pro）品种，以剂量依赖性方式比较蜂蜜水样品的体外抗炎作用。使用不同浓度蜂蜜水对红细胞进行膜稳定试验来评估体外抗炎活性。材料与方法。本研究针对选定的蜂蜜品种对暴露于热和低渗裂解的红细胞进行研究，并将膜损伤的抑制作用与标准药物乙酰水杨酸进行比较。
在体外红细胞模型溶血中，膜损伤均呈浓度依赖性受到抑制。低渗溶液诱导的损伤在10至50mg/ml时被蜂蜜水样品以升序抑制，抑制率从8.25%至97.76%，标准药物乙酰水杨酸在100μg/ml浓度时显示溶血保护率为96.09%。在热诱导溶血模型中，蜂蜜水样品在10至50mg/ml时，所有样品在外部应激条件下均表现出保护特性，保护率从0.44%至21.23%，乙酰水杨酸在100μg/ml浓度时显示保护率为39.38%。在这些品种中，PFf在50mg/ml时对低渗溶液诱导的裂解（97.76%）和热诱导的溶血（21.23%）表现出最高的保护特性。
根据这些研究数据得出结论，该模型具有显著的抗炎作用。未来需开展研究以确定蜂蜜中起作用的分子及其作用机制。
