Mine Disaster Prevention and Control Key Laboratory of Education Ministry of China, Shandong University of Science and Technology, Qingdao 266590, China; College of Electronics, Communications, and Physics, Shandong University of Science and Technology, Qingdao 266590, PR China.
College of Electronics, Communications, and Physics, Shandong University of Science and Technology, Qingdao 266590, PR China.
Biosens Bioelectron. 2017 May 15;91:465-471. doi: 10.1016/j.bios.2016.12.063. Epub 2016 Dec 30.
Monitoring blood coagulation is an important issue in the surgeries and the treatment of cardiovascular diseases. In this work, we reported a novel strategy for the blood coagulation monitoring based on a micro-electromechanical film bulk acoustic resonator. The resonator was excited by a lateral electric field and operated under the shear mode with a frequency of 1.9GHz. According to the apparent step-ladder curves of the frequency response to the change of blood viscoelasticity, the coagulation time (prothrombin time) and the coagulation kinetics were measured with the sample consumption of only 1μl. The procoagulant activity of thromboplastin and the anticoagulant effect of heparin on the blood coagulation process were illustrated exemplarily. The measured prothrombin times showed a good linear correlation with R=0.99969 and a consistency with the coefficient of variation less than 5% compared with the commercial coagulometer. The proposed film bulk acoustic sensor, which has the advantages of small size, light weight, low cost, simple operation and little sample consumption, is a promising device for miniaturized, online and automated analytical system for routine diagnostics of hemostatic status and personal health monitoring.
监测血液凝固是手术和心血管疾病治疗中的一个重要问题。在这项工作中，我们报道了一种基于微机电薄膜体声波谐振器的新型血液凝固监测策略。该谐振器由横向电场激励，以 1.9GHz 的剪切模式工作。根据血液粘弹性变化对频率响应的明显阶梯曲线，可以测量凝血时间（凝血酶原时间）和凝血动力学，样品消耗量仅为 1μl。血栓形成酶原的促凝活性和肝素对血液凝固过程的抗凝作用被举例说明。与商用凝血仪相比，所测量的凝血酶原时间具有很好的线性相关性（R=0.99969），且与变异系数小于 5%的一致性。该薄膜体声波传感器具有体积小、重量轻、成本低、操作简单、样品消耗少等优点，是一种用于微创、在线和自动分析系统的有前途的设备，用于常规止血状态诊断和个人健康监测。
