Chen Quan, Zhu Zhiling, Wang Jun, Lopez Analette I, Li Siheng, Kumar Amit, Yu Fei, Chen Haoqing, Cai Chengzhi, Zhang Lijuan
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China; Department of Chemistry, University of Houston, Houston, TX 77204, United States.
Department of Chemistry, University of Houston, Houston, TX 77204, United States.
Acta Biomater. 2017 Mar 1;50:353-360. doi: 10.1016/j.actbio.2017.01.011. Epub 2017 Jan 6.
Bacterial interference is an alternative strategy to fight against device-associated bacterial infections. Pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. In this work, biofilms formed by probiotic Escherichia coli strain Nissle 1917 (EcN) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in the digestive system, such as feeding tubes and voice prostheses. We have shown that EcN can form stable biofilms on silicone substrates, particularly those modified with a biphenyl mannoside derivative. These biofilms greatly reduced the colonization by pathogenic Enterococcus faecalis in Lysogeny broth (LB) for 11days.
Bacterial interference is an alternative strategy to fight against device-associated bacterial infections. Pursuing this strategy, we use non-pathogenic bacteria to form a biofilm that serves as a live, protective barrier against pathogen colonization. Herein, we report the first use of preformed probiotic E. coli Nissle 1917 biofilms on the mannoside-presenting silicone substrates to prevent pathogen colonization. The biofilms serve as a live, protective barrier to fence off the pathogens, whereas current antimicrobial/antifouling coatings are subjected to gradual coverage by the biomass from the rapidly growing pathogens in a high-nutrient environment. It should be noted that E. coli Nissle 1917 is commercially available and has been used in many clinical trials. We also demonstrated that this probiotic strain performed significantly better than the non-commercial, genetically modified E. coli strain that we previously reported.
细菌干扰是对抗与设备相关的细菌感染的一种替代策略。采用这种策略时，非致病性细菌生物膜被用作一种活的保护屏障，以防止病原体定植。在这项工作中，研究了由益生菌大肠杆菌Nissle 1917株（EcN）形成的生物膜对与硅基导尿管以及消化系统中使用的留置导管（如饲管和语音假体）相关感染进行长期细菌干扰的潜力。我们已经表明，EcN能够在硅基底物上形成稳定的生物膜，特别是那些用联苯甘露糖苷衍生物修饰的底物。这些生物膜在11天内显著减少了溶原肉汤（LB）中致病性粪肠球菌的定植。
细菌干扰是对抗与设备相关的细菌感染的一种替代策略。采用这种策略时，我们使用非致病性细菌形成生物膜，作为防止病原体定植的活的保护屏障。在此，我们报告首次在呈现甘露糖苷的硅基底物上使用预先形成的益生菌大肠杆菌Nissle 1917生物膜来防止病原体定植。生物膜作为活的保护屏障来阻挡病原体，而目前的抗菌/防污涂层在高营养环境中会逐渐被快速生长的病原体的生物量覆盖。应当指出的是，大肠杆菌Nissle 1917是可商购的，并且已经在许多临床试验中使用。我们还证明，这种益生菌菌株的表现明显优于我们之前报道的非商业性转基因大肠杆菌菌株。
