Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Sci Rep. 2017 Jan 10;7:40587. doi: 10.1038/srep40587.
The efficient production of antimicrobial peptides (AMPs) for clinical applications has attracted the attention of the scientific community. To develop a novel microbial cell factory for the efficient biosynthesis of a cecropin A-melittin mutant (CAM-W), a recombinant Bacillus subtilis WB700 expression system was genetically modified with a novel vector, including a fusion gene encoding CAM-W, the autoprotease EDDIE and the signal peptide SacB under the control of the maltose-inducible promoter P. A total of 159 mg of CAM-W was obtained from 1 L of fermentation supernatant. The purified CAM-W showed a consistent size with the expected molecular weight of 3.2 kDa. Our findings suggest that this novel expression system can be used as a powerful tool for the efficient production of CAM-W.
抗菌肽（AMPs）的高效生产引起了科学界的关注。为了开发一种新型的微生物细胞工厂，用于高效生物合成 Cecropin A-Melitlin 突变体（CAM-W），我们对重组枯草芽孢杆菌 WB700 表达系统进行了基因修饰，使用了一种新型载体，其中包括融合基因 CAM-W、自蛋白酶 EDDIE 和信号肽 SacB，它们受麦芽糖诱导型启动子 P 的控制。从 1L 发酵上清液中获得了 159mg 的 CAM-W。纯化后的 CAM-W 与预期的 3.2kDa 分子量一致。我们的研究结果表明，这种新型表达系统可以作为高效生产 CAM-W 的有力工具。
