a Department of Biotechnology , Motilal Nehru National Institute of Technology , Allahabad , Uttar Pradesh , India.
Crit Rev Food Sci Nutr. 2018 Jul 3;58(10):1715-1734. doi: 10.1080/10408398.2016.1276048. Epub 2017 Jul 21.
Biotechnology embraces various physical and chemical phenomena toward advancement of health diagnostics. Toward such advancement, detection of toxins plays an important role. Toxins produce severe health impacts on consumption with high mortality associated in acute cases. The most prominent route of infection and intoxication is through food matrices. Therefore, rapid detection of toxins at low concentrations is the need of modern diagnostics. Lateral flow immunoassays are one of the emergent and popularly used rapid detection technology developed for detecting various kinds of analytes. This review thus focuses on recent advancements in lateral flow immunoassays for detecting different toxins in agricultural food. Appropriate emphasis was given on how the labels, recognition elements, or detection strategy has laid an impact on improvement in immunochromatographic assays for toxins. The paper also discusses the gradual change in sensitivities and specificities of assays in accordance with the method of food processing used. The review concludes with the major challenges faced by this technology and provides an outlook and insight of ideas to improve it in the future.
生物技术利用各种物理和化学现象来促进健康诊断的发展。在这一发展过程中，毒素检测起着重要的作用。毒素的摄入会对健康造成严重影响，在急性病例中与高死亡率相关。感染和中毒的最主要途径是通过食物基质。因此，需要现代诊断技术来快速检测低浓度的毒素。侧向流动免疫分析是新兴的和广泛使用的快速检测技术之一，用于检测各种分析物。因此，本综述重点介绍了侧向流动免疫分析在检测农业食品中不同毒素方面的最新进展。适当强调了标签、识别元件或检测策略如何对免疫层析分析检测毒素的改进产生影响。本文还讨论了根据所用的食品加工方法，检测的灵敏度和特异性逐渐变化的情况。本文最后总结了该技术面临的主要挑战，并对未来改进该技术提供了展望和思路。
