Sohrabi Hessamaddin, Majidi Mir Reza, Khaki Pegah, Jahanban-Esfahlan Ali, de la Guardia Miguel, Mokhtarzadeh Ahad
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Compr Rev Food Sci Food Saf. 2022 Mar;21(2):1868-1912. doi: 10.1111/1541-4337.12913. Epub 2022 Feb 22.
Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs-based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.
纳米技术中最近引入的各种化学物质和一些物理现象使科学家能够在食品科学领域开发有用的设备。关于此类发展，检测食源性病原体细菌现在是一个重要问题。这类细菌在食用食物后已显示出严重的健康影响，并且与急性病例相关的死亡率很高。中毒和感染的最主要途径是通过食物基质。因此，当前诊断中需要在低浓度下快速识别食源细菌病原体。横向流动分析（LFA）是已被确定用于识别各种类型分析物的紧急且普遍应用的快速识别方法之一。因此，本综述着重介绍了基于LFA的平台在检测各种食源性病原体细菌方面的最新进展，如沙门氏菌、李斯特菌、大肠杆菌、布鲁氏菌、志贺氏菌、金黄色葡萄球菌、肉毒梭菌和霍乱弧菌。文中特别强调了标记物、检测元件或程序在使用LFA评估各种细菌的最新进展中所产生的影响。此外，还讨论了与应用的食品加工技术相一致的检测方法特异性和灵敏度的改进。最后，得出结论以突出通过该方法面临的主要挑战，并为其未来的进一步发展提供观点和见解。
