Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, PR China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, PR China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, PR China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, PR China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, PR China.
Talanta. 2017 Apr 1;165:167-175. doi: 10.1016/j.talanta.2016.12.042. Epub 2016 Dec 21.
Graphene oxide (GO) and carboxylated GO were used as labels for lateral flow immunoassays, instead of the conventionally used colloidal gold and colored latex labels. A sensor is demonstrated that enables fast screening for aflatoxin B (AFB) as a model analyte using the antibody-GO complex as the recognition element. The visual limit of detection and cut-off value for AFB are 0.3 and 1ng/mL, respectively. It is shown that GO and carboxylated GO are viable black labels for use in lateral flow assays, one typical advantage being the saving cost (compared to the use of colloidal gold). Qualitative results are achieved within 15min, and the analytical results were in good agreement with the reference LC MS/MS method. The method was successfully applied to the on-site determination of AFB in agricultural products. In our perception, it opens new possibilities for the screening of other toxins by lateral flow immunoassays using GO and carboxylated GO as labels.
氧化石墨烯（GO）和羧化氧化石墨烯被用作侧向流动免疫分析的标记物，而不是传统使用的胶体金和彩色乳胶标记物。展示了一种传感器，该传感器使用抗体-GO 复合物作为识别元件，可快速筛选黄曲霉毒素 B（AFB）作为模型分析物。AFB 的视觉检测限和截止值分别为 0.3 和 1ng/mL。结果表明，GO 和羧化 GO 是可用于侧向流动分析的可行的黑色标记物，其一个典型优势是节省成本（与使用胶体金相比）。在 15 分钟内即可获得定性结果，并且分析结果与参考 LC-MS/MS 方法吻合良好。该方法成功应用于农产品中 AFB 的现场测定。在我们看来，它为使用 GO 和羧化 GO 作为标记物通过侧向流动免疫分析筛选其他毒素开辟了新的可能性。
