Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
Biosens Bioelectron. 2017 May 15;91:482-488. doi: 10.1016/j.bios.2016.12.072. Epub 2017 Jan 3.
In this work, a real-time assay for highly sensitive, label-free, multiplexed electrical detection of lung cancer biomarkers was developed by using silicon nanowire field-effect (SiNW-FET) devices. Highly responsive SiNW arrays were fabricated using a CMOS-compatible anisotropic self-stop etching technique with mass reproducibility and low cost character. The SiNW nanosensor was integrated with PDMS microfluidic device, which allows rapid analyte delivery, makes the analysis to be conducted using exceedingly small samples and enables potential multiplexed detection. The nanowire arrays allowed highly selective and sensitive multiplexed detection of microRNA (miRNA)-126 and CEA. Due to high surface-to-volume ratio that the nanowire dimensions confer, the detection floor of single molecule was achieved. The potential utility in identifying clinical samples for early diagnosis of cancer was demonstrated by analyzing biomarkers in clinical related samples. The developed nanosensor with capability for multiplexed real-time monitoring of biomarkers with high sensitivity and selectivity in clinically relevant samples is highly attractive for diagnosis and treatment of cancer and other diseases.
本工作采用硅纳米线场效应（SiNW-FET）器件，开发了一种用于高灵敏度、无标记、多重肺癌生物标志物电检测的实时分析方法。通过具有高重复性和低成本特性的 CMOS 兼容各向异性自停止刻蚀技术，制造出了响应性强的 SiNW 阵列。SiNW 纳米传感器与 PDMS 微流控器件集成在一起，允许快速分析物输送，使用极少的样品进行分析，并实现潜在的多重检测。纳米线阵列可高度选择性和灵敏地检测 microRNA（miRNA）-126 和 CEA。由于纳米线尺寸赋予的高表面积与体积比，实现了单分子的检测下限。通过分析临床相关样本中的生物标志物，证明了该方法在识别临床样本以进行癌症早期诊断方面的潜在应用。该纳米传感器具有在临床相关样本中进行多重实时、高灵敏度和选择性生物标志物监测的能力，非常适合癌症和其他疾病的诊断和治疗。
