Galicia-Moreno Marina, Rosique-Oramas Dorothy, Medina-Avila Zaira, Álvarez-Torres Tania, Falcón Dalia, Higuera-de la Tijera Fátima, Béjar Yadira L, Cordero-Pérez Paula, Muñoz-Espinosa Linda, Pérez-Hernández José Luis, Kershenobich David, Gutierrez-Reyes Gabriela
HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico; Departamento de Farmacología, Facultad de Medicina Mexicali, UABC, Mexicali, BC, Mexico.
HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
Oxid Med Cell Longev. 2016;2016:9370565. doi: 10.1155/2016/9370565. Epub 2016 Dec 15.
Alcohol is the most socially accepted addictive substance worldwide, and its metabolism is related with oxidative stress generation. The aim of this work was to evaluate the role of oxidative stress in alcoholic liver cirrhosis (ALC). This study included 187 patients divided into two groups: ALC, classified according to Child-Pugh score, and a control group. We determined the levels of reduced and oxidized glutathione (GSH and GSSG) and the GSH/GSSG ratio by an enzymatic method in blood. Also, protein carbonyl and malondialdehyde (MDA) content were estimated in serum. MDA levels increased in proportion to the severity of damage, whereas the GSH and GSSG levels decreased and increased, respectively, at different stages of cirrhosis. There were no differences in the GSH/GSSG ratio and carbonylated protein content between groups. We also evaluated whether the active consumption of or abstinence from alcoholic beverages affected the behavior of these oxidative markers and only found differences in the MDA, GSH, and GSSG determination and the GSH/GSSG ratio. Our results suggest that alcoholic cirrhotic subjects have an increase in oxidative stress in the early stages of disease severity and that abstinence from alcohol consumption favors the major antioxidant endogen: GSH in patients with advanced disease severity.
酒精是全球范围内社会接受度最高的成瘾性物质，其代谢与氧化应激的产生有关。这项工作的目的是评估氧化应激在酒精性肝硬化（ALC）中的作用。本研究纳入了187例患者，分为两组：根据Child-Pugh评分分类的ALC组和对照组。我们通过酶法测定了血液中还原型和氧化型谷胱甘肽（GSH和GSSG）的水平以及GSH/GSSG比值。此外，还测定了血清中的蛋白质羰基和丙二醛（MDA）含量。MDA水平与损伤严重程度成正比，而在肝硬化的不同阶段，GSH水平下降，GSSG水平上升。两组之间的GSH/GSSG比值和羰基化蛋白质含量没有差异。我们还评估了酒精饮料的主动饮用或戒酒是否会影响这些氧化标志物的变化，结果仅发现MDA、GSH、GSSG测定值和GSH/GSSG比值存在差异。我们的结果表明，酒精性肝硬化患者在疾病严重程度的早期阶段氧化应激增加，而戒酒有利于晚期疾病严重程度患者体内主要的内源性抗氧化剂：GSH。
