Maidana S S, Delgado F, Vagnoni L, Mauroy A, Thiry E, Romera S
Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917 (C1033AAJ), Ciudad Autónoma de Buenos Aires, Argentina; Cátedra de Inmunogenética, Facultad de Ciencias exactas, Químicas y Naturales, Universidad de Morón, Morón, Buenos Aires, Argentina.
Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA) , Castelar, Buenos Aires , Argentina.
Vet Rec Open. 2016 Dec 12;3(1):e000162. doi: 10.1136/vetreco-2015-000162. eCollection 2016.
In the present work, controlled experimental infection and transmission studies in domestic cattle () and water buffaloes () were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (BuHV1). Two bovine and two buffalo calves were infected with BuHV1 (20287N isolate) by intranasal aerosolisation. Two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission. All experimentally inoculated animals showed viral infection and respiratory clinical signs. BuHV1 experimentally infected calves showed intermittent viral excretion between 2 days and 18 days postinfection (dpi) with a maximum titre of excretion of 10 TCID/ml and moderate rhinitis between 2 dpi and 20 dpi. BuHV1 experimentally inoculated buffaloes showed mild respiratory signs, which consisted mainly of serous nasal secretions during the infection period. Sentinel calves showed mucosal specific IgG antibodies at seven days postcontact. Viral DNA was detected by PCR and sequencing in both buffaloes and sentinel calves, which could be associated with latency. In conclusion, this study showed the susceptibility of cattle to BuHV1 after both experimental infection and contact with infected buffaloes. These data increase the scarce knowledge on the pathogenesis in natural host and the susceptibility of cattle to BuHV1 experimental infection.
在本研究中，我们对家牛（）和水牛（）进行了对照实验性感染和传播研究，以研究水泡性口炎病毒1型（BuHV1）在体内的行为。通过鼻内雾化，用BuHV1（20287N分离株）感染了两头牛犊和两头水牛犊。两头哨兵牛未接受病毒攻击，但与感染的水牛一起饲养，以评估水平传播。所有实验接种的动物均表现出病毒感染和呼吸道临床症状。实验感染BuHV1的犊牛在感染后2天至18天（dpi）之间出现间歇性病毒排泄，排泄的最高滴度为10 TCID/ml，在2 dpi至20 dpi之间出现中度鼻炎。实验接种BuHV1的水牛表现出轻度呼吸道症状，主要表现为感染期间的浆液性鼻分泌物。哨兵犊牛在接触后7天出现粘膜特异性IgG抗体。通过PCR和测序在水牛和哨兵犊牛中均检测到病毒DNA，这可能与潜伏有关。总之，本研究表明，牛在实验感染和与感染水牛接触后对BuHV1易感。这些数据增加了我们对天然宿主发病机制以及牛对BuHV1实验感染易感性的稀缺认识。
