Yang Tianshu, Zhao Zeping, Liu Tianqi, Zhang Ziwei, Wang Pengzu, Xu Shiwen, Lei Xin Gen, Shan Anshan
Northeast Agricultural University, Harbin, P. R. China.
Department of Animal Science, Cornell University, Ithaca, NY, USA.
Oncotarget. 2017 Feb 21;8(8):13428-13439. doi: 10.18632/oncotarget.14550.
The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines. The concentration of ROS was elevated (P < 0.05) in obese swine fed on a low Se diet (less than 0.03 mg/kg Se) compared to control swine. The protein levels of Nrf2 and its downstream target genes were depressed during Se deficiency and high-energy intake. The mRNA levels of 16 selenoproteins were significantly decreased (P < 0.05) in the Se-deficient group and Se-deficient, high-energy group compared to the control group. However, the mRNA levels of 13 selenoproteins in peripheral blood neutrophils were upregulated in high energy group, except TrxR1, SelI and SepW. In summary, these data indicated that a Se-deficient, high-energy diet inhibits the Nrf2 pathway and its regulation of oxidative stress, and prompted a pleiotropic mechanism that suppresses phagocytosis.
硒缺乏与高能量之间相互作用的机制仍然有限。本研究的目的是确定缺硒的高能量饮食是否会诱导氧化应激，下调Nrf2通路以及中性粒细胞的吞噬功能障碍。我们检测了中性粒细胞的吞噬活性、活性氧生成、Nrf2及其下游靶基因的蛋白质水平，以及25种硒蛋白、热休克蛋白和细胞因子的mRNA水平。使用细胞因子ELISA试剂盒检测血清细胞因子。与对照猪相比，以低硒饮食（硒含量低于0.03 mg/kg）喂养的肥胖猪中活性氧浓度升高（P < 0.05）。在缺硒和高能量摄入期间，Nrf2及其下游靶基因的蛋白质水平降低。与对照组相比，缺硒组和缺硒高能量组中16种硒蛋白的mRNA水平显著降低（P < 0.05）。然而，除TrxR1、SelI和SepW外，高能量组外周血中性粒细胞中13种硒蛋白的mRNA水平上调。总之，这些数据表明，缺硒的高能量饮食会抑制Nrf2通路及其对氧化应激的调节，并引发一种抑制吞噬作用的多效性机制。
