长链非编码RNA SPRY4-IT1对骨肉瘤细胞生物学行为的影响
Effects of long non-coding RNA SPRY4-IT1 on osteosarcoma cell biological behavior.
作者信息
Xu Jin, Ding Ren, Xu Yaozeng
机构信息
Department of Orthopedics, Baoshan District Shanghai Integrated Traditional Chinese and Western Medicine Hospital Shanghai, China.
Department of Orthopedics, The First Affiliated Hospital of Soochow University Suzhou, China.
出版信息
Am J Transl Res. 2016 Dec 15;8(12):5330-5337. eCollection 2016.
Abstract
Recent findings indicate that long noncoding RNAs (lncRNAs) were dysregulated in many kinds of tumors including osteosarcoma (OS). SPRY4-IT1 has been recently revealed as oncogenic regulator in various cancers, while its clinical value and potential function in OS are still unknown. To investigate the role of SPRY4-IT1 in OS, we evaluated the expression SPRY4-IT1 in OS tissues and cell lines, and investigated the effect of SPRY4-IT1 siRNA on cell proliferation, migration and invasion of OS . Our result showed that SPRY4-IT1 was upregulated in OS tissues. Further experiments revealed that SPRY4-IT1 knockdown significantly inhibited OS cells proliferation by causing G1 arrest and promoting apoptosis. Furthermore, inhibitory effects of SPRY4-IT1 on cell migration and invasion were partly associated with EMT process. In conclusion, these data suggest that SPRY4-IT1 could be an oncogene for OS, and may be served as a candidate target for new therapies in human OS.
摘要
最近的研究结果表明，长链非编码RNA（lncRNAs）在包括骨肉瘤（OS）在内的多种肿瘤中表达失调。SPRY4-IT1最近被揭示为多种癌症中的致癌调节因子，但其在骨肉瘤中的临床价值和潜在功能仍不清楚。为了研究SPRY4-IT1在骨肉瘤中的作用，我们评估了SPRY4-IT1在骨肉瘤组织和细胞系中的表达，并研究了SPRY4-IT1 siRNA对骨肉瘤细胞增殖、迁移和侵袭的影响。我们的结果显示，SPRY4-IT1在骨肉瘤组织中上调。进一步的实验表明，敲低SPRY4-IT1可通过导致G1期阻滞和促进凋亡显著抑制骨肉瘤细胞增殖。此外，SPRY4-IT1对细胞迁移和侵袭的抑制作用部分与上皮-间质转化（EMT）过程有关。总之，这些数据表明SPRY4-IT1可能是骨肉瘤的一个癌基因，并且可能成为人类骨肉瘤新疗法的候选靶点。
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