Crouse D A, Perry G A, Murphy B O, Sharp J G
Department of Anatomy, University of Nebraska Medical Center, Omaha 68105.
J Anat. 1989 Feb;162:53-65.
In this study we have examined the morphology and steroid sensitivity of proximal colonic lymphoid tissue in the Fisher 344 rat. A time course study was conducted in which groups of animals were injected subcutaneously with hydrocortisone sodium succinate (125 mg/kg body weight) and killed on Days 0-4. Thymus, jejunal and ileal Peyer's patches and proximal colonic lymphoid tissue were excised, weighed and processed for histological analysis. The results showed that the maximum cytoreductive effects of the hydrocortisone were evident on Day 2. Thymus and proximal colonic lymphoid tissue weight decreased to 5 and 18% of the control values respectively, before returning towards control values over the next two days. In contrast, jejunal and ileal Peyer's patch weights were unaltered. A dose response experiment was conducted using the same endpoints. Rats were injected subcutaneously with hydrocortisone at 60, 120, 200 and 300 mg/kg body weight and killed on Day 2. The results of this experiment showed that the proximal colonic lymphoid tissue, like thymus, responded with a dose-dependent loss of tissue weight. The spleen and Peyer's patches showed only a slight weight decrease compared to the control. These data showed that the response of proximal colonic lymphoid tissue to steroids was more similar to that of thymus, a primary lymphoid tissue, than to other secondary lymphoid tissues. Finally, grafts of fetal proximal colon under the kidney capsule of syngeneic adults supported the development of this lymphoid aggregate in the absence of luminal antigenic stimulation. These results suggest that the development and functional contribution of proximal colonic lymphoid tissue to the immune system warrants a more detailed examination.
在本研究中，我们检测了Fisher 344大鼠近端结肠淋巴组织的形态学及对类固醇的敏感性。进行了一项时间进程研究，将动物分组，皮下注射琥珀酸钠氢化可的松（125 mg/kg体重），并在第0 - 4天处死。切除胸腺、空肠和回肠派伊尔结以及近端结肠淋巴组织，称重并进行组织学分析。结果显示，氢化可的松的最大细胞减少效应在第2天最为明显。胸腺和近端结肠淋巴组织重量分别降至对照值的5%和18%，随后在接下来的两天内恢复至对照值。相比之下，空肠和回肠派伊尔结的重量未发生改变。使用相同的终点进行了剂量反应实验。大鼠皮下注射体重60、120、200和300 mg/kg的氢化可的松，并在第2天处死。该实验结果表明，近端结肠淋巴组织与胸腺一样，其组织重量随剂量依赖性减少。与对照组相比，脾脏和派伊尔结仅显示轻微的重量减轻。这些数据表明，近端结肠淋巴组织对类固醇的反应与作为初级淋巴组织的胸腺更为相似，而与其他次级淋巴组织不同。最后，将胎儿近端结肠移植到同基因成年大鼠的肾被膜下，在无腔内抗原刺激的情况下，支持了这种淋巴聚集物的发育。这些结果表明，近端结肠淋巴组织对免疫系统的发育及功能贡献值得进行更详细的研究。
