Aslam Khan Muhammad Umar, Raza Mohsin Ali, Mehboob Hassan, Abdul Kadir Mohammed Rafiq, Abd Razak Saiful Izwan, Shah Saqlain A, Iqbal Muhammad Zahir, Amin Rashid
Department of Polymer Engineering and Technology, University of the Punjab 54590 Lahore Pakistan
School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia 81300 Skudai Johor Malaysia.
RSC Adv. 2020 Nov 6;10(66):40529-40542. doi: 10.1039/d0ra07446b. eCollection 2020 Nov 2.
The excellent biocompatible and osteogenesis characteristics of porous scaffolds play a vital role in bone regeneration. In this study, we have synthesized polymeric hybrid nanocomposites free-radical polymerization from carrageenan/acrylic-acid/graphene/hydroxyapatite. Porous hybrid nanocomposite scaffolds were fabricated through a freeze-drying method to mimic the structural and chemical composition of natural bone. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and water contact-angle studies were carried-out for functional groups, surface morphology and hydrophilicity of the materials, followed by biodegradation and swelling analysis. The cell viability, cell culture and proliferation were evaluated against mouse pre-osteoblast () cell lines using neutral red dye assay. The cell adherence and proliferation studies were determined by SEM. Physical characterization including optimum porosity and pore size (49.75% and 0.41 × 10 μm), mechanical properties (compression strength 8.87 MPa and elastic modulus 442.63 MPa), swelling (70.20% at 27 °C and 77.21% at 37 °C) and biodegradation (23.8%) were performed. The results indicated CG--AAc-3 with a high optical density and better cell viability. Hence, CG--AAc-3 was found to be more efficient for bone regeneration with potential applications in fractured bone regeneration.
多孔支架优异的生物相容性和成骨特性在骨再生中起着至关重要的作用。在本研究中，我们通过卡拉胶/丙烯酸/石墨烯/羟基磷灰石的自由基聚合反应合成了聚合物杂化纳米复合材料。采用冷冻干燥法制备多孔杂化纳米复合支架，以模拟天然骨的结构和化学成分。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和水接触角研究对材料的官能团、表面形态和亲水性进行了分析，随后进行了生物降解和溶胀分析。使用中性红染料法对小鼠前成骨细胞系的细胞活力、细胞培养和增殖进行了评估。通过SEM对细胞黏附和增殖进行了研究。进行了包括最佳孔隙率和孔径（49.75%和0.41×10μm）、力学性能（抗压强度8.87MPa和弹性模量442.63MPa）、溶胀（27℃时为70.20%，37℃时为77.21%）和生物降解（23.8%）在内的物理表征。结果表明，CG- -AAc-3具有较高的光密度和较好的细胞活力。因此，发现CG- -AAc-3在骨折骨再生中具有更有效的骨再生潜力应用。
